Moriya T, Sakamoto K, Sasano H, Kawanaka M, Sonoo H, Manabe T, Ito J
Department of Pathology, Tohoku University Hospital, Sendai, Japan.
Mod Pathol. 2000 Jan;13(1):13-8. doi: 10.1038/modpathol.3880004.
We examined Ki-67, p53, p21, and p27 immunolocalization in 43 cases of apocrine lesions of the breast and correlated these findings with histologic parameters to understand their biologic significance. Twenty cases were benign, 1 case was borderline, and 22 cases were diagnosed as malignant, including 9 intraductal and 13 invasive apocrine carcinomas. Both the ratio of Ki-67-positive cases (17 of 21 [88.9%] versus 1 of 19 [5.3%]; P < .001) and the Ki-67 labeling index of positive cases examined (15.0% versus 2.7%; P < .005) were significantly higher in malignant than in benign apocrine lesions. None of the benign or borderline cases was immunohistochemically positive for p53, but 15 of 22 malignant cases (68.2%) demonstrated p53 (P < .001). In addition, the ratio of p53-positive cases was significantly higher in high nuclear grade cases (11 of 13 [84.6%]) than in intermediate nuclear grade cases (4 of 9 [44.4%]; P < .05). P53 immunoreactivity was also positively correlated with the nuclear grade of carcinoma cases examined in this study. Neither p21 nor p27 demonstrated any correlation with histologic parameters or findings of the apocrine lesions. Results of these studies suggest that Ki-67 and p53 may be good markers for differentiation between benign and malignant breast apocrine lesions.
我们检测了43例乳腺大汗腺病变中Ki-67、p53、p21和p27的免疫定位，并将这些结果与组织学参数相关联，以了解它们的生物学意义。20例为良性，1例为交界性，22例被诊断为恶性，包括9例导管内癌和13例浸润性大汗腺癌。Ki-67阳性病例的比例（21例中的17例[88.9%]对比19例中的1例[5.3%]；P <.001）以及所检测的阳性病例的Ki-67标记指数（15.0%对比2.7%；P <.005）在恶性大汗腺病变中均显著高于良性病变。良性或交界性病例中没有一例p53免疫组化呈阳性，但22例恶性病例中有15例（68.2%）显示p53阳性（P <.001）。此外，p53阳性病例的比例在高核级病例（13例中的11例[84.6%]）中显著高于中核级病例（9例中的4例[44.4%]；P <.05）。在本研究中检测的癌病例中，p53免疫反应性也与核级呈正相关。p21和p27均未显示与大汗腺病变的组织学参数或结果有任何相关性。这些研究结果表明，Ki-67和p53可能是区分乳腺良性和恶性大汗腺病变的良好标志物。
