Warr G W, DeLuca D, Marchalonis J J
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2476-80. doi: 10.1073/pnas.73.7.2476.
Membrane immunoglobulin (Ig) of splenocytes and thymocytes of the goldfish, Carassius auratus, was demonstrated by indirect fluorescent-antibody techniques. Observations on shedding and resynthesis indicated that the thymocyte Ig was endogenously produced. The lymphocyte surface proteins were radioiodinated using the lactoperoxidase-catalyzed reaction, and the labeled Ig molecules were isolated by specific precipitation and analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The IgM-like membrane Igs of splenocytes and thymocytes were shown to differ in their ease of solubilization with nonionic detergent, and in the sodium dodecyl sulfate/electrophoretic mobility of their heavy chains. The significance of these observations for the evolution of T-cell recognition is discussed.
采用间接荧光抗体技术证实了金鱼（Carassius auratus）脾细胞和胸腺细胞的膜免疫球蛋白（Ig）。关于脱落和再合成的观察表明胸腺细胞Ig是内源性产生的。利用乳过氧化物酶催化反应对淋巴细胞表面蛋白进行放射性碘化，通过特异性沉淀分离标记的Ig分子，并通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳进行分析。结果显示，脾细胞和胸腺细胞的IgM样膜Ig在非离子去污剂溶解的难易程度以及重链的十二烷基硫酸钠/电泳迁移率方面存在差异。讨论了这些观察结果对T细胞识别进化的意义。
