酶促碘化作用。一种检测正常和肿瘤性淋巴细胞可及表面蛋白的探针。
Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes.
作者信息
Marchalonis J J, Cone R E, Santer V
出版信息
Biochem J. 1971 Oct;124(5):921-7. doi: 10.1042/bj1240921.
Abstract
- Radioactive iodide was covalently bound to living cells from normal mouse spleen and a variety of lymphoid tumours by a system consisting of lactoperoxidase, hydrogen peroxide and iodide. 2. About 3x10(5)-6x10(5) molecules of [(125)I]iodide/cell could be incorporated without affecting cell viability. 3. Electron-micrographic radioautography showed that the radioactive label was associated with the outer surfaces of the cells. 4. Radioiodinated proteins were solubilized in 9m-urea-0.2m-mercaptoethanol and analysed by gel-filtration and disc electrophoresis. 5. Comparison of distinct tumour lines by disc electrophoresis showed qualitative and quantitative differences in protein distribution patterns.
摘要
- 通过由乳过氧化物酶、过氧化氢和碘化物组成的系统，放射性碘化物与正常小鼠脾脏及多种淋巴瘤的活细胞共价结合。2. 每细胞可掺入约3×10⁵ - 6×10⁵个[(¹²⁵)I]碘化物分子，且不影响细胞活力。3. 电子显微镜放射自显影显示放射性标记与细胞外表面相关。4. 放射性碘化蛋白质在9m尿素 - 0.2m巯基乙醇中溶解，并通过凝胶过滤和圆盘电泳进行分析。5. 通过圆盘电泳对不同肿瘤细胞系进行比较，结果显示蛋白质分布模式存在定性和定量差异。
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