Secombes C J, van Groningen J J, Egberts E
Immunology. 1983 Jan;48(1):165-75.
Lymphoid cell populations in various organs of the carp Cyprinus carpio L. were investigated using a series of mouse monoclonal antibodies raised against carp thymocytes and carp serum Ig. Clones have been designated as Ig+T+, Ig+T- or Ig-T+ on the basis of the reactivity with thymocytes and serum Ig in the enzyme-linked immunosorbent assay (ELISA) screening. Their reaction to the lymphoid organs of carp was investigated on cryostat sections and cytocentrifuge slides using immunoperoxidase techniques. IG+T+ clones could be subdivided into those that stained reticular fibres around blood vessels in various organs (R+) and those that did not (R-). The former stained most thymocytes and most peripheral lymphocytes as well as plasma cells whereas the latter did not stain cortical thymocytes and some peripheral lymphocytes. IG+T- clones were negative for thymocytes but positive for plasma cells and a certain population of peripheral lymphocytes. Ig-T+ clones reacted similarly to Ig+T+R- clones. It is concluded that fish lymphoid cell populations can be distinguished based upon differences in cell surface and/or cytoplasmic determinants. The monoclonal antibodies described can be used for further structural analysis of the determinants and for functional separation of T- and B-like cells in the 'lower' vertebrates.
利用一系列针对鲤鱼胸腺细胞和鲤鱼血清免疫球蛋白（Ig）制备的小鼠单克隆抗体，对鲤鱼（Cyprinus carpio L.）各器官中的淋巴细胞群体进行了研究。在酶联免疫吸附测定（ELISA）筛选中，根据与胸腺细胞和血清Ig的反应性，将克隆分为Ig+T+、Ig+T-或Ig-T+。使用免疫过氧化物酶技术，在低温恒温器切片和细胞离心涂片上研究了它们对鲤鱼淋巴器官的反应。Ig+T+克隆可细分为在各器官中对血管周围网状纤维染色的克隆（R+）和不染色的克隆（R-）。前者能使大多数胸腺细胞、大多数外周淋巴细胞以及浆细胞染色，而后者不能使皮质胸腺细胞和一些外周淋巴细胞染色。Ig+T-克隆对胸腺细胞呈阴性，但对浆细胞和一定数量的外周淋巴细胞呈阳性。Ig-T+克隆的反应与Ig+T+R-克隆相似。得出的结论是，鱼类淋巴细胞群体可根据细胞表面和/或细胞质决定簇的差异来区分。所述单克隆抗体可用于进一步分析决定簇的结构，以及在“低等”脊椎动物中对T样细胞和B样细胞进行功能分离。
