一种使用胶原凝胶微滴包埋培养法的结直肠癌体外化学敏感性测试。
An in vitro chemosensitivity test for colorectal cancer using collagen-gel droplet embedded cultures.
作者信息
Araki Y, Isomoto H, Matsumoto A, Kaibara A, Yasunaga M, Hayashi K, Yatsugi H, Yamauchi K
机构信息
Department of Surgery, Kurume University Medical Center, Japan.
出版信息
Kurume Med J. 1999;46(3-4):163-6. doi: 10.2739/kurumemedj.46.163.
This study evaluated in vitro assay for chemosensitivity test using a collagen-gel droplet embedded culture drug sensitivity test (CD-DST) for colorectal cancer. CD-DST was performed in 24 patients with Dukes B, C colorectal cancer. Primary cultures of tumor cell samples from 87.5% (21/24) patients with colorectal cancer were successful. The efficacy rates assessed by CD-DST of five anticancer drugs evaluated were: 60.4% for adriamycin, 58.9% for etoposide, 56.7% for cisplatin, 53.4% for 5-fluorouracil, for 31.1% for mitomycin C, and 9.5% for vindesine. The present study demonstrated the clinical usefulness of CD-DST in evaluating the response to chemotherapy in colorectal cancer.
本研究使用胶原凝胶微滴包埋培养药物敏感性试验（CD-DST）对结直肠癌进行体外化学敏感性试验评估。对24例Dukes B、C期结直肠癌患者进行了CD-DST。87.5%（21/24）的结直肠癌患者肿瘤细胞样本原代培养成功。所评估的五种抗癌药物经CD-DST评估的有效率分别为：阿霉素60.4%，依托泊苷58.9%，顺铂56.7%，5-氟尿嘧啶53.4%，丝裂霉素C 31.1%，长春地辛9.5%。本研究证明了CD-DST在评估结直肠癌化疗反应方面的临床实用性。
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