Gonzalez MJ, Cabrera A
Department of Genetics, ETSIAM, University of Cordoba, Spain.
Genome. 1999 Dec;42(6):1194-200. doi: 10.1139/g99-028.
Total genomic Hordeum chilense DNA probe was hybridized to somatic chromosome spreads of Triticum aestivum 'Chinese Spring' and to four advanced tritordeum lines, the latter being the fertile amphiploid between H. chilense and durum wheat (2n = 6x = 42, AABBH(ch)H(ch)). The probe hybridized strongly to the B-genome chromosomes and to one or two bands on the A-genome chromosomes present in both wheat and tritordeum alloploids. Bands on chromosomes 1D, 2D, and 7D from hexaploid wheat were also detected. Genomic H. chilense DNA probe identified 16 chromosome pairs of the chromosome complement of hexaploid wheat and all A- and B-genome chromosomes present in the tritordeum amphiploids. The in situ hybridization patterns observed correspond to those previously reported in wheat by both N-banding and in situ hybridization with the GAA-satellite sequence (Pedersen and Langridge 1997), allowing the identification of these chromosomes. Variation among the tritordeum amphiploids for hybridization sites on chromosomes 2A, 4A, 6A, 7A, 4B, 5B, and 7B was observed. Despite of this polymorphism, all lines shared the general banding pattern. When used as probe, total H. chilense genomic DNA labeled the H. chilense chromosomes over their lengths allowing the identification of 14 H. chilense chromosomes present in the tritordeum amphiploids. In addition, chromosome-specific telomeric, interstial, and centromeric hybridization sites were observed. These hybridization sites coincide with N-banded regions in H. chilense allowing the identification of the individual H. chilense chromosomes in one of the amphiploid. The N-banded karyotypes of H. chilense (accessions H1 and H7) are presented.
将总基因组大麦草（Hordeum chilense）DNA探针与普通小麦‘中国春’的体细胞染色体铺片以及四个高级三属杂种品系进行杂交，后者是大麦草与硬粒小麦之间的可育双二倍体（2n = 6x = 42，AABBH(ch)H(ch)）。该探针与B基因组染色体以及小麦和三属杂种异源多倍体中A基因组染色体上的一条或两条带强烈杂交。还检测到了六倍体小麦1D、2D和7D染色体上的带。基因组大麦草DNA探针鉴定出了六倍体小麦染色体组中的16对染色体以及三属杂种双二倍体中所有的A和B基因组染色体。观察到的原位杂交模式与之前通过N带和与GAA卫星序列的原位杂交在小麦中报道的模式一致（Pedersen和Langridge，1997），从而能够鉴定这些染色体。观察到三属杂种双二倍体在2A、4A、6A、7A、4B、5B和7B染色体上杂交位点的变异。尽管存在这种多态性，但所有品系都具有共同的带型模式。当用作探针时，总大麦草基因组DNA在其长度上标记了大麦草染色体，从而能够鉴定出三属杂种双二倍体中存在的14条大麦草染色体。此外，还观察到了染色体特异性的端粒、间质和着丝粒杂交位点。这些杂交位点与大麦草中的N带区域一致，从而能够在其中一个双二倍体中鉴定出单个大麦草染色体。展示了大麦草（种质H1和H7）的N带核型。
