通过荧光原位杂交鉴定涉及小麦、大麦和智利大麦染色体的基因组间易位

Identification of intergenomic translocations involving wheat, Hordeum vulgare and Hordeum chilense chromosomes by FISH.

作者信息

Prieto P, Ramírez M C, Ballesteros J, Cabrera A

机构信息

Instituto de Agricultura Sostenible (CSIC), Apdo. 4084, 14080 Córdoba, Spain.

出版信息

Hereditas. 2001;135(2-3):171-4. doi: 10.1111/j.1601-5223.2001.t01-1-00171.x.

DOI:10.1111/j.1601-5223.2001.t01-1-00171.x

PMID:12152330

Abstract

Intergenomic translocations between wheat, Hordeum chilense and Hordeum vulgare have been obtained in tritordeum background. Advanced lines from the crosses between three disomic chromosome addition lines for chromosome 2Hv, 3Hv, and 4Hv of barley (Hordeum vulgare) in Triticum aestivum cv. Chinese Spring (CS) and hexaploid tritordeum (2n = 6x = 42, AABBHchHch) were analyzed. Multicolor FISH using both genomic DNA from H. chilense and H. vulgare were used to establish the presence and numbers of H. vulgare introgressions into tritordeum. Interspecific H. vulgare/H. chilense and intergeneric wheat/H. vulgare and wheat/H. chilense translocations were identified. Frequencies of plants containing different kinds of intergenomic translocations between chromosome arms are presented. These lines can be useful for introgressing into tritordeum characters of interest from H. vulgare.

摘要

在三倍体小麦背景下已获得小麦、智利大麦和大麦之间的基因组间易位。对普通小麦中国春品种（CS）中大麦（大麦）2Hv、3Hv和4Hv染色体的三个二体染色体附加系与六倍体三倍体小麦（2n = 6x = 42，AABBHchHch）杂交的后代进行了分析。使用来自智利大麦和大麦的基因组DNA进行多色荧光原位杂交，以确定大麦渗入三倍体小麦中的情况及其数量。鉴定出了种间大麦/智利大麦以及属间小麦/大麦和小麦/智利大麦的易位。给出了含有不同类型染色体臂间基因组间易位的植株频率。这些品系可用于将大麦中感兴趣的性状导入三倍体小麦。