Green M R
Howard Hughes Medical Institute Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, MA 01605, USA.
Trends Biochem Sci. 2000 Feb;25(2):59-63. doi: 10.1016/s0968-0004(99)01527-3.
Transcription of eukaryotic structural genes requires the assembly of RNA polymerase II and the general transcription factors (GTFs) on the promoter to form a pre-initiation complex (PIC). Among these, TFIID is the major sequence-specific DNA-binding component; the other GTFs enter the PIC primarily through protein-protein interactions. TFIID is composed of the TATA-box-binding protein (TBP) and multiple TBP-associated factors (TAFIIs). Unexpectedly, TAFIIs have also been found in other multi-subunit complexes involved in transcription. Whereas TBP is a general transcription factor, a variety of in vivo studies have demonstrated that TAFIIs are highly promoter selective. Here I review studies on the role of TAFIIs in genome-wide transcription and their mechanism of action.
真核生物结构基因的转录需要RNA聚合酶II和通用转录因子（GTFs）在启动子上组装，以形成前起始复合物（PIC）。其中，TFIID是主要的序列特异性DNA结合成分；其他GTFs主要通过蛋白质-蛋白质相互作用进入PIC。TFIID由TATA盒结合蛋白（TBP）和多个TBP相关因子（TAFIIs）组成。出乎意料的是，在参与转录的其他多亚基复合物中也发现了TAFIIs。虽然TBP是一种通用转录因子，但各种体内研究表明，TAFIIs具有高度的启动子选择性。在此，我综述了关于TAFIIs在全基因组转录中的作用及其作用机制的研究。
