Sala A, Recio M C, Giner R M, Máñez S, Ríos J L
Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Burjassot, Spain.
Life Sci. 2000;66(2):PL35-40. doi: 10.1016/s0024-3205(99)00578-0.
The activity of the Santolina chamaecyparissus methanol extract was tested against the phospholipase A2 (PLA2)-induced mouse paw edema and in vitro inhibition of PLA2 activity. After fractionation, only the dichloromethane extract was active against the PLA2 in vitro test. In addition, it reduced the edema induced by arachidonic acid, and by 12-O-tetradecanoylphorbol-13-acetate in a multidose test. After chromatography on silicagel and gel filtration on Sephadex, and using an in vitro anti-PLA2 assay-guided process, we have isolated and identified from the dichloromethane extract the flavone nepetin and four sesquiterpenes.
对圣托里尼柏木叶甲醇提取物进行了抗磷脂酶A2（PLA2）诱导的小鼠爪肿胀活性及体外抑制PLA2活性的测试。分馏后，仅二氯甲烷提取物在体外PLA2测试中具有活性。此外，在多剂量试验中，它减轻了花生四烯酸和12-O-十四烷酰佛波醇-13-乙酸酯诱导的肿胀。在硅胶柱色谱和葡聚糖凝胶过滤后，采用体外抗PLA2测定指导的方法，我们从二氯甲烷提取物中分离并鉴定出黄酮类化合物荆芥苷和四种倍半萜。
