Ali Abuzer, Ali Amena, Husain Warsi Musarrat, Ahmad Wasim, Tahir Abu
Department of Pharmacognosy, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Saudi J Biol Sci. 2021 Aug;28(8):4575-4580. doi: 10.1016/j.sjbs.2021.04.060. Epub 2021 Apr 30.
is an important medicinal plant growing in the Mediterranean region and has been reported as a potent anti-inflammatory, antibacterial, antioxidant, and antifungal agent. The purpose of the current research is to identify the chemical constituents in ethyl acetate extract (EAE) from the leaves of , and to evaluate antidiabetic, and anticancer activity. Chemical constituents of EAE were identified by GC-MS, and the antidiabetic activity was evaluated by α-glucosidase inhibition assay. The anticancer activity was assessed by Epidermal Growth Factor Receptor (EGFR) expression in human breast cancer cell line (MCF7) by using quantitative RT-PCR method. GC-MS analysis of EAE of yielded 44 compounds. Tetrapentacontane (27.15%), eicosyl acetate (8.40%), 2-methylhexacosane (6.87%), and -pentadecanol (5.44%) were found as major chemical constituents. The EAE of showed concentration dependant inhibition of α-glucosidase enzyme and the IC value (IC 110 ± 4.25 µg/mL) was found comparable with standard acarbose (IC 105 ± 3.74 µg/mL). The real-time qRT-PCR results showed that the EGFR protein () in human breast cancer cell line (MCF7) was negatively expressed with a value of -0.69297105 after treatment with EAE (100 µg/mL). The study results are suggesting the possible use of in the management of diabetes, and human breast cancer.
是一种生长在地中海地区的重要药用植物，据报道具有强效抗炎、抗菌、抗氧化和抗真菌作用。本研究的目的是鉴定该植物叶片乙酸乙酯提取物（EAE）中的化学成分，并评估其抗糖尿病和抗癌活性。通过气相色谱 - 质谱联用（GC - MS）鉴定EAE的化学成分，通过α - 葡萄糖苷酶抑制试验评估抗糖尿病活性。使用定量逆转录 - 聚合酶链反应（RT - PCR）方法通过人乳腺癌细胞系（MCF7）中表皮生长因子受体（EGFR）的表达评估抗癌活性。对该植物EAE的GC - MS分析产生了44种化合物。发现二十九烷（27.15%）、二十烷基乙酸酯（8.40%）、2 - 甲基二十六烷（6.87%）和十五烷醇（5.44%）为主要化学成分。该植物的EAE对α - 葡萄糖苷酶表现出浓度依赖性抑制，其半数抑制浓度值（IC 110 ± 4.25 μg/mL）与标准阿卡波糖（IC 105 ± 3.74 μg/mL）相当。实时定量RT - PCR结果表明，在用EAE（100 μg/mL）处理后人乳腺癌细胞系（MCF7）中的EGFR蛋白（）呈负表达，值为 - 0.69297105。研究结果表明该植物在糖尿病和人类乳腺癌管理中可能具有应用价值。
