核30S核糖核蛋白亚复合物中mRNA序列的检测
Detection of mRNA sequences in nuclear 30S ribonucleoprotein subcomplexes.
作者信息
Kinniburgh A J, Martin T E
出版信息
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2725-9. doi: 10.1073/pnas.73.8.2725.
Abstract
RNA from nuclear 30S ribonucleoprotein (RNP) complexes of mouse ascites cells has been shows to contain sequences homologous to poly(A) + mRNA by its ability to hybridize with complementary DNA prepared from poly(A) + mRNA template. Analysis of the hybridization kinetics of poly(A) + mRNA with its own complementary DNA revealed several abundancy classes. The total complexity of poly(A) + mRNA from ascites cells was estimated to be approximately 30,000 sequences of average molecular weight (6 X 10(5)). When the hybridization reaction of 30S RNP-RNA with mRNA-specific cDNA was compared to the homologous reaction the majority, and most probably all, of the poly(A) + mRNA sequences were found to be present in the RNA. The kinetics of hybridization suggest that 10-15% of the RNA in this RNP complex is homologous to poly(A) + mRNA. The 30S RNP subcomplexes therefore contain nuclear poly(A) + mRNA sequences as well as the bulk of heterogeneous RNA.
摘要
小鼠腹水细胞核30S核糖核蛋白（RNP）复合物中的RNA，通过与从多聚腺苷酸（poly(A)）+ mRNA模板制备的互补DNA杂交的能力，已显示含有与poly(A) + mRNA同源的序列。对poly(A) + mRNA与其自身互补DNA杂交动力学的分析揭示了几个丰度类别。腹水细胞中poly(A) + mRNA的总复杂性估计约为平均分子量（6×10⁵）的30,000个序列。当将30S RNP - RNA与mRNA特异性cDNA的杂交反应与同源反应进行比较时，发现大部分（很可能是所有）poly(A) + mRNA序列都存在于该RNA中。杂交动力学表明，该RNP复合物中10 - 15%的RNA与poly(A) + mRNA同源。因此，30S RNP亚复合物既包含细胞核poly(A) + mRNA序列，也包含大部分不均一RNA。
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