Hahn S T, O'Connor J M
School of Natural and Complementary Medicine, Southern Cross University, Lismore, NSW, Australia.
Toxicon. 2000 Jan;38(1):79-89. doi: 10.1016/s0041-0101(99)00135-x.
Bullrout envenomation is known to cause intense pain. Crude bullrout venom and venom fractions were assessed for protease, hyaluronidase, phospholipase and hemolytic activities, reactivity with stonefish antivenom, lethality to brine shrimp and ability to elicit pain in human subjects. Compared with venom obtained from frozen specimens, live fish venom-milking techniques rendered greater venom potency and improved storage characteristics. Although mild proteolytic and hemolytic activity was observed, crude venom demonstrated no hyaluronidase or phospholipase A2 activity, did not affect brine shrimp, or show antigenicity with stonefish antivenom. A single venom protein isolated from bullrout venom is attributed with causing pain in human subjects. The sensations elicited by this novel algesic protein are consistent with chemical stimulation of polymodal nociceptors.
据了解，杜父鱼蜇伤会引发剧痛。对杜父鱼粗毒液及毒液组分进行了蛋白酶、透明质酸酶、磷脂酶和溶血活性评估，检测了其与石鱼抗蛇毒血清的反应性、对卤虫的致死性以及在人体引发疼痛的能力。与从冷冻标本中获取的毒液相比，活鱼取毒技术能产生更强的毒液效力，并改善储存特性。尽管观察到轻微的蛋白水解和溶血活性，但粗毒液未表现出透明质酸酶或磷脂酶A2活性，对卤虫无影响，也未与石鱼抗蛇毒血清显示出抗原性。从杜父鱼毒液中分离出的一种单一毒液蛋白被认为是导致人体疼痛的原因。这种新型致痛蛋白引发的感觉与多模式伤害感受器的化学刺激一致。
