Oguiur N, Camargo M E, da Silva A R, Horton D S
Instituto Butantan, Biotecnology Center, São Paulo, SP, Brazil.
Toxicon. 2000 Mar;38(3):443-8. doi: 10.1016/s0041-0101(99)00157-9.
Intraspecific variation in Crotalus durissus terrificus venom composition was studied in relation to crotamine activity. Crotamine induces paralysis in extension of hind legs of mice and myonecrosis in skeletal muscle cells. To determine whether the venom of crotamine-negative rattlesnake contains a quantity of myotoxin incapable of inducing paralysis, we have developed a very sensitivity immunological assay method, an enzyme-linked immunoabsorbent assay (ELISA), capable of detecting 0.6 ng of purified crotamine. The parallel-lines analysis of ELISA data showed to be useful because it shows the reliability of the experimental conditions. A variation in the amount of myotoxin in the crotamine-positive venom was observed, but not less than 0.1 mg of crotamine per mg of venom. It was not possible to detect it in crotamine-negative venom even at high venom concentrations.
研究了巴西矛头蝮毒液成分的种内变异与响尾蛇毒素活性的关系。响尾蛇毒素可导致小鼠后腿伸展麻痹以及骨骼肌细胞发生肌坏死。为了确定不含响尾蛇毒素的响尾蛇毒液中是否含有一定量无法诱导麻痹的肌毒素，我们开发了一种灵敏度极高的免疫分析方法，即酶联免疫吸附测定法（ELISA），该方法能够检测出0.6纳克纯化的响尾蛇毒素。ELISA数据的平行线分析显示是有用的，因为它表明了实验条件的可靠性。在含响尾蛇毒素的毒液中观察到了肌毒素含量的变化，但每毫克毒液中响尾蛇毒素含量不少于0.1毫克。即使在高毒液浓度下，也无法在不含响尾蛇毒素的毒液中检测到它。
