Shashidharamurthy R, Jagadeesha D K, Girish K S, Kemparaju K
Department of Studies in Biochemistry, University of Mysore, Manasagangothri, India.
Mol Cell Biochem. 2002 Jan;229(1-2):93-101. doi: 10.1023/a:1017972511272.
Indian cobra (Naja naja naja) venom obtained from three different geographical regions was studied in terms of electrophoretic pattern, biochemical and pharmacological activities. SDS-PAGE banding pattern revealed significant variation in the protein constituents of the three regional venoms. The eastern venom showed highest indirect hemolysis and hyaluronidase activity. In contrast, western and southern venoms were rich in proteolytic activity. All the three regional venoms were devoid of p-tosyl-L-arginine methyl ester hydrolysing activity. The eastern venom was found to be most lethal among the three regional venoms. The lethal potency varied as eastern > western > southern regional venoms. In addition, all the three regional venoms showed marked variations in their ability to induce symptoms/signs of neurotoxicity, myotoxicity, edema and effect on plasma coagulation process. Polyclonal antiserum prepared against the venom of eastern region cross-reacted with both southern and western regional venoms, but varied in the extent of cross-reactivity by ouchterlony immunodiffusion and ELISA.
对从三个不同地理区域获取的印度眼镜蛇（眼镜蛇指名亚种）毒液进行了电泳图谱、生化和药理活性方面的研究。SDS-PAGE条带图谱显示，这三种区域毒液的蛋白质成分存在显著差异。东部毒液表现出最高的间接溶血和透明质酸酶活性。相比之下，西部和南部毒液富含蛋白水解活性。所有三种区域毒液均缺乏对甲苯磺酰-L-精氨酸甲酯水解活性。发现东部毒液在三种区域毒液中致死性最强。致死效力的变化顺序为东部＞西部＞南部区域毒液。此外，所有三种区域毒液在诱导神经毒性、肌毒性、水肿症状/体征以及对血浆凝血过程的影响方面表现出明显差异。针对东部区域毒液制备的多克隆抗血清与南部和西部区域毒液均发生交叉反应，但通过免疫双扩散和ELISA法检测，交叉反应程度有所不同。
