节杆菌属Q36菌株海藻糖操纵子中编码糖原脱支酶的基因的克隆与核苷酸序列分析
Cloning and nucleotide sequence of a gene encoding a glycogen debranching enzyme in the trehalose operon from Arthrobacter sp. Q36.
作者信息
Maruta K, Kubota M, Fukuda S, Kurimoto M
机构信息
Hayashibara Biochemical Laboratories, Inc., 7-7 Amase-minami machi, Okayama, Japan.
出版信息
Biochim Biophys Acta. 2000 Feb 9;1476(2):377-81. doi: 10.1016/s0167-4838(99)00253-8.
A gene located just upstream of the treYZ operon was isolated from Arthrobacter sp. strain Q36. The gene, designated treX, encoded an 823-amino acid protein. The amino acid sequence of the protein had 50% identity with the TreX protein (isoamylase) from Sulfolobus acidocaldarius ATCC 33909 which has a treZXY operon on the genome. We suggest that Arthrobacter treX is an isoamylase gene, and that it is a component of a treXYZ operon.
从节杆菌属菌株Q36中分离出一个位于treYZ操纵子上游的基因。该基因命名为treX，编码一个含有823个氨基酸的蛋白质。该蛋白质的氨基酸序列与嗜酸嗜热栖热菌ATCC 33909的TreX蛋白（异淀粉酶）具有50%的同一性，嗜酸嗜热栖热菌的基因组上有一个treZXY操纵子。我们认为节杆菌treX是一个异淀粉酶基因，并且它是treXYZ操纵子的一个组成部分。
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