Maruta K, Mitsuzumi H, Nakada T, Kubota M, Chaen H, Fukuda S, Sugimoto T, Kurimoto M
Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
Biochim Biophys Acta. 1996 Dec 6;1291(3):177-81. doi: 10.1016/s0304-4165(96)00082-7.
Trehalose biosynthesis genes, treZ, treX and treY, encoding maltooligosyltrehalose trehalohydrolase (TreZ), glycogen debranching enzyme (TreX), and maltooligosyltrehalose synthase (TreY) have been cloned from the thermophilic archaebacterium Sulfolobus acidocaldarius ATCC33909. The amino-acid sequences deduced from treZ, treX and treY are composed of 556, 713 and 720 amino-acid residues, respectively. TreZ and TreY are 33-40% homologous to the corresponding enzymes from Arthrobacter sp. Q36. We have proposed that the biosynthesis of trehalose in Sulfolobus occurs via the actions of the three enzymes encoded by treZXY.
海藻糖生物合成基因treZ、treX和treY，分别编码麦芽寡糖基海藻糖海藻糖水解酶（TreZ）、糖原脱支酶（TreX）和麦芽寡糖基海藻糖合酶（TreY），已从嗜热古细菌嗜酸热硫化叶菌ATCC33909中克隆出来。从treZ、treX和treY推导的氨基酸序列分别由556、713和720个氨基酸残基组成。TreZ和TreY与节杆菌属Q36相应酶的同源性为33%-40%。我们提出，硫化叶菌中海藻糖的生物合成是通过treZXY编码的三种酶的作用来实现的。
