Maruta K, Hattori K, Nakada T, Kubota M, Sugimoto T, Kurimoto M
Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
Biochim Biophys Acta. 1996 Feb 9;1289(1):10-3. doi: 10.1016/0304-4165(95)00139-5.
A 5.1-kbp genomic DNA fragment was cloned from trehalose-producing bacterium Arthrobacter sp. strain Q36. Sequence analysis of the DNA fragment revealed two open reading frames of 2325 and 1794 bp, encoding maltooligosyltrehalose synthase (TreY) and maltooligosyltrehalose trehalohydrolase (TreZ), respectively. The 3' end of treY overlaps with the 5' end of treZ by one nucleotide, and it is suggested that treYZ constitutes and operon. The deduced amino acid sequences of both enzymes have several regions common to amylolytic enzymes belonging to an 'alpha-amylase family'.
从产海藻糖的节杆菌属菌株Q36中克隆出一个5.1千碱基对的基因组DNA片段。对该DNA片段的序列分析显示有两个分别为2325和1794碱基对的开放阅读框，它们分别编码麦芽寡糖基海藻糖合酶（TreY）和麦芽寡糖基海藻糖海藻糖水解酶（TreZ）。treY的3'端与treZ的5'端有一个核苷酸重叠，提示treYZ构成一个操纵子。两种酶推导的氨基酸序列有几个区域与属于“α-淀粉酶家族”的淀粉分解酶相同。
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