Penn A S, Chang H W, Lovelace R E, Niemi W, Miranda A
Ann N Y Acad Sci. 1976;274:354-76. doi: 10.1111/j.1749-6632.1976.tb47697.x.
Acetylcholine receptors (AChR), purified from electric eel and Torpedo by affinity chromatography using a synthetic quaternary ammonium ligand, bound 10 nmole alpha-bungarotoxin (alpha-BuTx) per mg of protein and demonstrated a common subunit. Rabbits, immunized with either eel or Torpedo/AChR, developed flaccid paralysis barely altered by anticholinesterases and died 48 hours after the first sign of paralysis. In paralyzed animals, repetitive stimulation at low rates induced a 50% to 90% decrement of evoked potentials, temporarily reversed by edrophonium. Extracellular and introcellular MEPP amplitudes were 40% to 50% of controls. Serum and isolated IgG formed single immunoprecipitin lines against the antigens. AChR-antibody complexes did not bind alpha-BuTx, whereas alpha-BuTx-AChR complexes bound antibody if the antibody was specific for that AChR. Torpedo and eel AChR showed partial idenity with both antisera. Rat diaphragms and eel electroplax incubated with antisera to Torpedo and eel AChR showed a 50%-60% reduction in carbamylcholine depolarization. These studies demonstrate differences between eel and Torpedo AChR induce a block in neuromuscular transmission in rabbits with features of myasthenia gravis.
通过使用合成季铵配体的亲和层析法从电鳗和电鳐中纯化得到的乙酰胆碱受体（AChR），每毫克蛋白质结合10纳摩尔的α-银环蛇毒素（α-BuTx），并显示出一个共同的亚基。用鳗鱼或电鳐/AChR免疫的兔子出现弛缓性麻痹，抗胆碱酯酶几乎不能改变这种麻痹状态，并且在出现麻痹的第一个迹象后48小时死亡。在麻痹的动物中，以低频率重复刺激诱发电位降低50%至90%，依酚氯铵可暂时逆转这种降低。细胞外和细胞内微小终板电位（MEPP）幅度为对照的40%至50%。血清和分离的免疫球蛋白G（IgG）针对抗原形成单一免疫沉淀线。AChR-抗体复合物不结合α-BuTx，而如果抗体对该AChR具有特异性，则α-BuTx-AChR复合物结合抗体。电鳐和鳗鱼AChR与两种抗血清都显示出部分同一性。用针对电鳐和鳗鱼AChR的抗血清孵育的大鼠膈肌和鳗鱼电板，氨甲酰胆碱去极化降低了50%至60%。这些研究表明鳗鱼和电鳐AChR之间的差异会在兔子中诱导神经肌肉传递阻滞，具有重症肌无力的特征。
