Lindstrom J
J Supramol Struct. 1976;4(3):389-403. doi: 10.1002/jss.400040310.
Immunochemical techniques for the study of acetylcholine receptors are described. Immunization of rabbits, rats, guinea pigs, and goats with acetylcholine receptor protein purified from Electrophorus electric organ tissue results in muscular weakness and death due to impaired neuromuscular transmission. Serum from immunized animals contains high concentrations of antibodies directed at receptors from the electric organ and low concentrations of antibodies directed at receptors from skeletal muscle. The detailed similarities between the disease of receptor-immunized animals "experimental autoimmune myasthenia gravis" (EAMG), and myasthenia gravis are compared. Reactions of antisera from animals with EAMG with receptor from Electrophorus and Torpedo are studied. Antireceptor antibodies in these antisera are directed predominantly and determinants other than the acetylcholine-binding site.
描述了用于研究乙酰胆碱受体的免疫化学技术。用从电鳗电器官组织中纯化的乙酰胆碱受体蛋白对兔、大鼠、豚鼠和山羊进行免疫，会因神经肌肉传递受损而导致肌肉无力和死亡。免疫动物的血清中含有高浓度针对电器官受体的抗体和低浓度针对骨骼肌受体的抗体。比较了受体免疫动物的疾病“实验性自身免疫性重症肌无力”（EAMG）与重症肌无力之间的详细相似之处。研究了患有EAMG的动物的抗血清与电鳗和电鳐受体的反应。这些抗血清中的抗受体抗体主要针对除乙酰胆碱结合位点以外的决定簇。
