Shirakura Y, Sugiyama T, Tanaka H, Taguchi T, Kawai S
Department of Orthopedic Surgery, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi, 755-8505, Japan.
Biochem Biophys Res Commun. 2000 Jan 27;267(3):752-5. doi: 10.1006/bbrc.1999.2027.
In order to examine the involvement of leptin in the ossification of spinal ligaments (OSL), the present study examined (i) serum levels of leptin and insulin in OSL patients and controls, (ii) serum leptin levels in children of OSL females with severe obesity, (iii) the expression of leptin receptor mRNA in human spinal ligaments, and (iv) effects of leptin on cultured human ligament cells. In the OSL females, serum leptin levels were significantly higher than those of the control females, and the levels were positively correlated to the serum insulin levels, while in the control females, there was a tendency of inverse correlation. The daughters of OSL females with severe obesity also had high serum leptin levels, although they had not developed OSL. The expression of leptin receptor mRNA was confirmed in the ligaments, but leptin did not influence the alkaline phosphatase activity nor procollagen type I carboxyl-terminal peptide content of the ligament cells. These findings suggest that leptin is involved genetically and indirectly with the pathogenesis of OSL in female patients.
为了研究瘦素在脊柱韧带骨化（OSL）中的作用，本研究检测了：（i）OSL患者和对照组血清中瘦素和胰岛素水平；（ii）重度肥胖的OSL女性患者子女的血清瘦素水平；（iii）人脊柱韧带中瘦素受体mRNA的表达；（iv）瘦素对培养的人韧带细胞的影响。在OSL女性患者中，血清瘦素水平显著高于对照组女性，且与血清胰岛素水平呈正相关，而在对照组女性中则呈负相关趋势。重度肥胖的OSL女性患者的女儿血清瘦素水平也较高，尽管她们尚未发生OSL。在韧带中证实了瘦素受体mRNA的表达，但瘦素并不影响韧带细胞的碱性磷酸酶活性和I型前胶原羧基末端肽含量。这些发现表明，瘦素在女性患者OSL的发病机制中存在遗传和间接关联。
