Handelman S L, Washburn F, Wopperer P
J Am Dent Assoc. 1976 Nov;93(5):967-70. doi: 10.14219/jada.archive.1976.0007.
Teeth in which caries had penetrated the pits and fissures were sealed with an ultraviolet-light-polymerized sealant. Samples of carious dentin were taken from teeth that were not sealed and from teeth that were sealed, up to two years after the sealant had been placed. The major reduction in viable microorganisms occurred during the first two weeks, and there was a gradual reduction in the total count thereafter. At the end of two years, there was a 2,000-fold decrease in the number of cultivable microorganisms. Preliminary clinical and radiographic findings suggest that there was no progression of the carious lesions, but further studies are needed before this technique can be considered an alternative to conventional procedures.
龋坏已侵入窝沟的牙齿用紫外线聚合封闭剂进行封闭。从不进行封闭的牙齿以及进行封闭的牙齿中采集龋损牙本质样本，采集时间为封闭剂放置后长达两年。在最初的两周内，存活微生物数量大幅减少，此后总数逐渐减少。在两年结束时，可培养微生物数量减少了2000倍。初步的临床和影像学检查结果表明龋损病变没有进展，但在该技术可被视为传统治疗方法的替代方案之前，还需要进一步研究。
