Jensen O E, Handelman S L
Scand J Dent Res. 1980 Oct;88(5):382-8. doi: 10.1111/j.1600-0722.1980.tb01243.x.
A total of 106 molars with occlusal caries were sealed with an autopolymerizing sealant. Samples of the carious dentin were obtained 1, 3, 7, 14 and 28 d, and 2, 4, 6, and 12 months after sealant placement. The samples were cultured on enriched nonselective medium, a streptococcal medium, a Streptococcus mutans medium, and a medium for acidogenic microorganisms. The bacterial counts were compared with control samples obtained from nonsealed occlusal carious lesions, half of which had acid conditioner applied to the occlusal surface for 1 min prior to sampling. The mean total viable counts decreased by approximately 99.9% during the 1-year study period, from 925.1 x 10(4) CFU/mg of sample to 0.9 x 10(4) CFU/mg of sample. Statistically, there was a very strong linear relationship (P less than 0.001) between the reduction in bacterial counts and time. A similar reduction was observed for the total streptococcal count and the S. mutans count. The etching procedure itself reduced the number of cultivable organisms by about 75%. Sealant material that was polymerized directly in culture media did not result in any inhibition of bacterial growth. Although slight fluctuations in the relative proportion of the microflora were observed at the different sampling intervals, there were no significant changes in the relative distribution of bacterial types with time.
共有106颗患有咬合面龋的磨牙用自凝封闭剂进行了封闭。在封闭剂放置后的1天、3天、7天、14天和28天，以及2个月、4个月、6个月和12个月获取龋坏牙本质样本。将样本接种于富集非选择性培养基、链球菌培养基、变形链球菌培养基和产酸微生物培养基上培养。将细菌计数与从未封闭的咬合面龋损获取的对照样本进行比较，其中一半对照样本在取样前在咬合面应用酸蚀剂1分钟。在为期1年的研究期间，平均总活菌数从925.1×10⁴CFU/mg样本降至0.9×10⁴CFU/mg样本，下降了约99.9%。从统计学上看，细菌计数的减少与时间之间存在非常强的线性关系（P<0.001）。链球菌总数和变形链球菌计数也观察到类似的减少。酸蚀操作本身使可培养微生物数量减少了约75%。直接在培养基中聚合的封闭剂材料未对细菌生长产生任何抑制作用。尽管在不同取样间隔观察到微生物群落相对比例有轻微波动，但细菌类型的相对分布随时间没有显著变化。
