Going R E, Loesche W J, Grainger D A, Syed S A
J Am Dent Assoc. 1978 Sep;97(3):455-62. doi: 10.14219/jada.archive.1978.0327.
Carious lesions that were covered with a pit and fissure sealant for five years yielded bacterial cultures that were predominantly negative. Sixteen of 18 test sites judged to have active caries in 1972 were found inactive in 1977; ten of 12 sites suspected to have caries in 1972 were deemed to have inactive caries in 1977. Sealant treatment resulted in an apparent 89% reversal from a caries-active to a caries-inactive state. These data confirm and extend previous observations that a limited number of cultivable organisms persist in some lesions but their numbers are few, and they do not appear capable of continuing the destruction of tooth structure.
用窝沟封闭剂覆盖龋损五年后，细菌培养结果大多为阴性。1972年判定为有活动性龋的18个测试部位中，1977年有16个被发现无活动性龋；1972年怀疑有龋的12个部位中，1977年有10个被认为是静止性龋。封闭剂治疗使龋活跃状态明显逆转至龋静止状态的比例达89%。这些数据证实并拓展了之前的观察结果，即一些龋损中存在数量有限的可培养微生物，但数量很少，且它们似乎无法继续破坏牙齿结构。
