Vogel J L, Kristie T M
Laboratory of Viral Diseases, National Institutes of Health, Building 4, Room 133, 4 Center Drive, Bethesda, MD 20892, USA.
EMBO J. 2000 Feb 15;19(4):683-90. doi: 10.1093/emboj/19.4.683.
Transcription of the herpes simplex virus 1 (HSV-1) immediate early (IE) genes is determined by multiprotein enhancer complexes. The core enhancer assembly requires the interactions of the POU-homeodomain protein Oct-1, the viral transactivator alphaTIF and the cellular factor C1 (HCF). In this context, the C1 factor interacts with each protein to assemble the stable enhancer complex. In addition, the IE enhancer cores contain adjacent binding sites for other cellular transcription factors such as Sp1 and GA-binding protein (GABP). In this study, a direct interaction of the C1 factor with GABP is demonstrated, defining the C1 factor as the critical coordinator of the enhancer complex assembly. In addition, mutations that reduce the GABP transactivation potential also impair the C1-GABP interaction, indicating that the C1 factor functions as a novel coactivator of GABP-mediated transcription. The interaction and coordinated assembly of the enhancer proteins by the C1 factor may be critical for the regulation of the HSV lytic-latent cycle.
单纯疱疹病毒1型（HSV-1）即刻早期（IE）基因的转录由多蛋白增强子复合物决定。核心增强子组装需要POU-同源结构域蛋白Oct-1、病毒反式激活因子αTIF和细胞因子C1（HCF）相互作用。在此背景下，C1因子与每种蛋白相互作用以组装稳定的增强子复合物。此外，IE增强子核心含有其他细胞转录因子如Sp1和GA结合蛋白（GABP）的相邻结合位点。在本研究中，证明了C1因子与GABP之间存在直接相互作用，将C1因子定义为增强子复合物组装的关键协调者。此外，降低GABP反式激活潜能的突变也会损害C1-GABP相互作用，表明C1因子作为GABP介导转录的新型共激活因子发挥作用。C1因子对增强子蛋白的相互作用和协同组装可能对HSV裂解-潜伏周期的调控至关重要。
