Hargrave M, Karunaratne A, Cox L, Wood S, Koopman P, Yamada T
Centre for Molecular and Cellular Biology, University of Queensland, Brisbane, Queensland, 4072, Australia.
Dev Biol. 2000 Mar 1;219(1):142-53. doi: 10.1006/dbio.1999.9581.
Cell-type diversity along the dorsoventral axis of the developing neural tube is influenced by factors secreted by groups of cells at the dorsal and ventral midline. Upon reception of these signals, precursor cells express specific sets of transcription factors which, in turn, play critical roles in cell-type specification. Here we report the cloning and characterization of Sox14, a novel and highly conserved member of the Sry-related Sox transcription factor gene family, in mouse and chick. Sox14 expression is restricted to a limited population of neurons in the developing brain and spinal cord of both species. Sox14 marks a subset of interneurons at a defined dorsoventral position adjacent to ventral motor neurons in the spinal cord. In vivo grafting of chick notochord tissue to ectopic positions adjacent to the developing spinal cord altered the expression domain of Sox14. Furthermore, expression of Sox14 in spinal cord explants was found to be regulated by Sonic hedgehog in a dose-dependent manner. These data implicate a novel class of transcription factors in dorsoventral cell-type specification in the spinal cord.
发育中神经管背腹轴上的细胞类型多样性受到背侧和腹侧中线细胞群分泌因子的影响。在前体细胞接收到这些信号后，它们会表达特定的转录因子组合，而这些转录因子反过来在细胞类型特化中发挥关键作用。在此，我们报告了小鼠和鸡中Sry相关Sox转录因子基因家族的一个新的高度保守成员Sox14的克隆和特性。Sox14的表达仅限于这两个物种发育中的脑和脊髓中的有限神经元群体。Sox14标记了脊髓中与腹侧运动神经元相邻的特定背腹位置的中间神经元亚群。将鸡脊索组织体内移植到发育中脊髓附近的异位位置，改变了Sox14的表达域。此外，发现脊髓外植体中Sox14的表达受音猬因子以剂量依赖方式调控。这些数据表明一类新的转录因子参与脊髓背腹细胞类型特化。
