Key Laboratory of Healthy Mariculture for East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, China.
National Demonstration Center for Experimental Aquatic Science and Technology Education, Jimei University, Xiamen, 361021, China.
Fish Physiol Biochem. 2022 Oct;48(5):1193-1207. doi: 10.1007/s10695-022-01110-3. Epub 2022 Aug 13.
As a member of the Sox gene family, Sox3 plays a vital role in gonadal development and gametogenesis. Nevertheless, the exact expression pattern of this gene in fish is still unknown. Here, we identified the Sox3 gene of Centropyge vrolikii, namely, Cv-Sox3. The Cv-Sox3 mRNA expression in the ovary and testis was detected by reverse transcription-polymerase chain reaction (RT-PCR) analysis, and the mRNA expression level of Cv-Sox3 in the ovary in the resting stage was significantly higher than that in other tissues. The phylogenetic tree and alignment of multiple sequences were constructed to analyze the evolutionary relationships of Cv-Sox3. Cv-Sox3 was relatively conserved in the evolution of teleost fish, indicating the importance and similarity of its function. The in situ hybridization results demonstrate that Cv-Sox3 was present in the follicle cells and cytoplasm of oocytes in the ovary of different stages, and the positive signals occurred in germ cells of the testis. After interfering with Cv-Sox3, the growth rate of ovarian cells in culture became slow, and the expression of ovary-bias-related genes Cyp19a and Foxl2 significantly increased. Meanwhile, the expression of testis-bias-related genes Dmrt1, Sox9, Cyp11a, Amh, and Sox8 significantly decreased. These results suggest that Cv-Sox3 gene might be expressed in the germ cells of male and female gonads during gonadal development. This study provides a precise expression pattern of Cv-Sox3 and demonstrates that Cv-Sox3 might play a significant role in the reproductive regulation of C. vrolikii. In this study, Sox3 of C. vrolikii (Cv-Sox3) was cloned to understand the expression pattern in the gonadal development, which is expressed in germ cells, involved in the process of gonadal development. The results demonstrated that Cv-Sox3 may play a significant role in the reproductive regulation of C. vrolikii.
作为 Sox 基因家族的一员， Sox3 在性腺发育和配子发生中起着至关重要的作用。然而，该基因在鱼类中的确切表达模式尚不清楚。在这里，我们鉴定了 Centropyge vrolikii 的 Sox3 基因，即 Cv-Sox3。通过逆转录聚合酶链反应（RT-PCR）分析检测到 Cv-Sox3 在卵巢和睾丸中的 mRNA 表达，在静止期卵巢中 Cv-Sox3 的 mRNA 表达水平显著高于其他组织。构建了系统进化树和多序列比对来分析 Cv-Sox3 的进化关系。Cv-Sox3 在硬骨鱼类的进化中相对保守，表明其功能的重要性和相似性。原位杂交结果表明，Cv-Sox3 存在于不同阶段卵巢滤泡细胞和卵母细胞质中，在睾丸生殖细胞中出现阳性信号。干扰 Cv-Sox3 后，培养的卵巢细胞生长速度变慢，卵巢偏倚相关基因 Cyp19a 和 Foxl2 的表达显著增加。同时，睾丸偏倚相关基因 Dmrt1、Sox9、Cyp11a、Amh 和 Sox8 的表达显著降低。这些结果表明，Cv-Sox3 基因可能在性腺发育过程中在雌雄生殖细胞中表达。本研究提供了 Cv-Sox3 的精确表达模式，并表明 Cv-Sox3 可能在 C. vrolikii 的生殖调控中发挥重要作用。在这项研究中，克隆了 C. vrolikii 的 Sox3（Cv-Sox3），以了解其在性腺发育中的表达模式，该基因在生殖细胞中表达，参与了性腺发育过程。研究结果表明，Cv-Sox3 可能在 C. vrolikii 的生殖调控中发挥重要作用。
