White J S, Astill M, Lawrence P J
Antimicrob Agents Chemother. 1979 Feb;15(2):204-8. doi: 10.1128/AAC.15.2.204.
Cephapirin was utilized to examine the interaction of beta-lactam antibiotics with growing Bacillus subtilis cells and the biological effects simultaneously produced. Saturation binding and quantitative cell death were observed at the cephapirin concentration of 0.1 mug/ml. Cephapirin bound to all penicillin-binding proteins except the d-alanine carboxypeptidase. A specific [(14)C]benzylpenicillin-binding assay was developed for the d-alanine carboxypeptidase. At the lowest saturating concentration of antibiotic (0.1 mug/ml), cephapirin inhibited formation of the d-alanine carboxypeptidase. Upon incubation with cephapirin, 18% of the membranous d-alanine carboxypeptidase was released into the media. The data suggest that beta-lactam antibiotics may affect the formation of bacterial cytoplasmic membranes in addition to their effect on cell wall synthesis.
头孢匹林被用于研究β-内酰胺类抗生素与生长中的枯草芽孢杆菌细胞的相互作用以及同时产生的生物学效应。在头孢匹林浓度为0.1微克/毫升时观察到饱和结合和定量细胞死亡。头孢匹林与除d-丙氨酸羧肽酶之外的所有青霉素结合蛋白结合。针对d-丙氨酸羧肽酶开发了一种特异性的[(14)C]苄青霉素结合测定法。在最低饱和抗生素浓度（0.1微克/毫升）下，头孢匹林抑制d-丙氨酸羧肽酶的形成。与头孢匹林孵育后，18%的膜结合d-丙氨酸羧肽酶释放到培养基中。数据表明，β-内酰胺类抗生素除了影响细胞壁合成外，还可能影响细菌细胞质膜的形成。
