Simkin G O, Tao J S, Levy J G, Hunt D W
QLT PhotoTherapeutics Inc., Vancouver, BC, Canada.
J Immunol. 2000 Mar 1;164(5):2457-62. doi: 10.4049/jimmunol.164.5.2457.
We have explored the effect of photodynamic therapy (PDT) with verteporfin on the induction and expression of contact hypersensitivity (CHS) to 2,4-dinitrofluorobenzene (DNFB) in normal mice and IL-10-deficient mice. Our results indicate that DNFB sensitized mice given PDT with verteporfin and whole body red light irradiation exhibited a significant reduction in CHS compared with control animals. Administration of rIL-12 reversed the effect(s) of PDT as did treatment of mice with anti-IL-10-neutralizing Ab. Knockout mice deficient in IL-10 were found to be resistant to the inhibitory effects of PDT. In vitro proliferative responses using spleen cells from DNFB-sensitized and PDT-treated mice showed a significantly lower response to DNBS as compared with cells from DNFB-sensitized mice or DNFB and PDT-treated IL-10-deficient mice. Finally, naive mice exposed to PDT exhibited an increase in skin IL-10 levels, which peaked between 72 and 120 h post-PDT. Together these data support the role of IL-10 as a key modulator in the inhibition of the CHS response by whole body PDT.
我们探究了维替泊芬光动力疗法（PDT）对正常小鼠和白细胞介素-10（IL-10）缺陷小鼠针对2,4-二硝基氟苯（DNFB）的接触性超敏反应（CHS）诱导及表达的影响。我们的结果表明，与对照动物相比，接受维替泊芬PDT及全身红光照射的DNFB致敏小鼠的CHS显著降低。给予重组白细胞介素-12（rIL-12）以及用抗IL-10中和抗体处理小鼠均可逆转PDT的作用。发现IL-10缺陷的基因敲除小鼠对PDT的抑制作用具有抗性。使用来自DNFB致敏且经PDT处理小鼠的脾细胞进行的体外增殖反应显示，与来自DNFB致敏小鼠或DNFB致敏且经PDT处理的IL-10缺陷小鼠的细胞相比，对二硝基苯磺酸（DNBS）的反应显著降低。最后，接受PDT的未致敏小鼠皮肤IL-10水平升高，在PDT后72至120小时达到峰值。这些数据共同支持IL-10作为全身PDT抑制CHS反应的关键调节因子的作用。
