Gottenbos B, van der Mei H C, Busscher H J
Department of Biomedical Engineering, University of Groningen, Bloemsingel 10, 9712 KZ, Groningen, The Netherlands.
J Biomed Mater Res. 2000 May;50(2):208-14. doi: 10.1002/(sici)1097-4636(200005)50:2<208::aid-jbm16>3.0.co;2-d.
The infection risk of biomaterials implants varies between different materials and is determined by an interplay of adhesion and surface growth of the infecting organisms. In this study, we compared initial adhesion and surface growth of Staphylococcus epidermidis HBH(2) 102 and Pseudomonas aeruginosa AK1 on poly(dimethylsiloxane), Teflon, polyethylene, polypropylene, polyurethane, poly(ethylene terephthalate), poly(methyl methacrylate), and glass. Initial adhesion was measured in situ in a parallel plate flow chamber with microorganisms suspended in phosphate-buffered saline, while subsequent surface growth was followed in full and in 20 times diluted growth medium. Initial adhesion of both bacterial strains was similar to all biomaterials. In full growth medium, generation times of surface growing S. epidermidis ranged from 17 to 38 min with no relation to wettability, while in diluted growth medium generation times increased from 44 to 98 min with increasing surface wettability. For P. aeruginosa no influence of surface wettability on generation times was observed, but generation times increased with decreasing desorption rates, maximal generation times being 47 min and minimal values down to 30 min. Generally, generation times of adhering bacteria were shorter than of planktonic bacteria. In conclusion, surface growth of initially adhering bacteria is influenced by biomaterials surface properties to a greater extent than initial adhesion.
生物材料植入物的感染风险因材料不同而有所差异，并且由感染生物体的黏附与表面生长之间的相互作用决定。在本研究中，我们比较了表皮葡萄球菌HBH(2) 102和铜绿假单胞菌AK1在聚二甲基硅氧烷、特氟龙、聚乙烯、聚丙烯、聚氨酯、聚对苯二甲酸乙二酯、聚甲基丙烯酸甲酯和玻璃上的初始黏附及表面生长情况。在平行板流动腔中，将微生物悬浮于磷酸盐缓冲盐水中原位测量初始黏附，而随后在完全生长培养基和20倍稀释的生长培养基中跟踪表面生长情况。两种菌株对所有生物材料的初始黏附情况相似。在完全生长培养基中，表面生长的表皮葡萄球菌的代时为17至38分钟，与润湿性无关，而在稀释生长培养基中，随着表面润湿性增加，代时从44分钟增加到98分钟。对于铜绿假单胞菌，未观察到表面润湿性对代时有影响，但代时随解吸速率降低而增加，最大代时为47分钟，最小值低至30分钟。一般来说，黏附细菌的代时比浮游细菌的代时短。总之，初始黏附细菌的表面生长比初始黏附受生物材料表面性质的影响更大。
