Kao W Katherine, Gagnon Patricia M, Vogel Joseph P, Chole Richard A
Department of Otolaryngology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, U.S.A.
Department of Molecular Microbiology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, U.S.A.
Laryngoscope. 2017 Jul;127(7):1655-1661. doi: 10.1002/lary.26499. Epub 2017 Mar 14.
Chronic, persistent infections complicate otologic procedures utilizing implantable devices such as cochlear implants or tympanostomy tubes. These infections are thought to be due to the establishment of microbial biofilms on implant surfaces. To address this issue, we hypothesized that surface charge modification may inhibit the formation of Pseudomonas aeruginosa biofilms on implant surfaces in vitro and in vivo.
We evaluated the effect of surface charge modification on bacterial biofilm formation by assessing the effect of the surface charge on bacterial adhesion in vitro and bacterial persistence in vivo.
To study the effect of surface charge in vitro, the surface wells in culture plates were modified using a layer-by-layer polyelectrolyte assembly method. Bacterial adherence was measured at 30-, 60-, and 120-minute intervals. To study the effect of surface charge modification in vivo, the surface of titanium microscrews was similarly modified and then surgically implanted into the dorsal calvaria of adult rats and inoculated with bacteria. Two weeks after implantation and inoculation, the number of bacteria remaining in vivo was evaluated.
Surface charge modification results in a significant decrease in adherence of bacteria in vitro. Surface charge modification of titanium microscrew implants also resulted in a significant decrease in P. aeruginosa recovered 2 weeks after surgical implantation.
Charge modification decreases the number of bacteria adherent to a surface in vitro and decreases the risk and severity of implant infection in an in vivo rat infection model. These results have promising biomedical applications.
NA. Laryngoscope, 127:1655-1661, 2017.
慢性持续性感染会使使用人工耳蜗或鼓膜造孔管等植入式装置的耳科手术变得复杂。这些感染被认为是由于微生物生物膜在植入物表面形成所致。为解决这一问题，我们假设表面电荷修饰可能在体外和体内抑制铜绿假单胞菌生物膜在植入物表面的形成。
我们通过评估表面电荷对体外细菌黏附和体内细菌存活的影响，来评估表面电荷修饰对细菌生物膜形成的作用。
为研究表面电荷在体外的作用，使用逐层聚电解质组装法对培养板中的表面孔进行修饰。每隔30分钟、60分钟和120分钟测量细菌黏附情况。为研究表面电荷修饰在体内的作用，对钛微螺钉表面进行类似修饰，然后通过手术植入成年大鼠的颅骨背侧并接种细菌。植入和接种两周后，评估体内残留的细菌数量。
表面电荷修饰导致体外细菌黏附显著减少。钛微螺钉植入物的表面电荷修饰还导致手术植入两周后回收的铜绿假单胞菌数量显著减少。
电荷修饰在体外减少了黏附在表面的细菌数量，并在体内大鼠感染模型中降低了植入物感染的风险和严重程度。这些结果具有良好的生物医学应用前景。
无。《喉镜》，2017年，第127卷，第1655 - 1661页。
