Barton A J, Sagers R D, Pitt W G
Department of Chemical Engineering, Brigham Young University, Provo, Utah 84602, USA.
J Biomed Mater Res. 1996 Mar;30(3):403-10. doi: 10.1002/(SICI)1097-4636(199603)30:3<403::AID-JBM15>3.0.CO;2-K.
The degradable polymers poly(orthoester) (POE), poly(L-lactic acid) (PLA), and the nondegradable polymers polysulfone (PSF), polyethylene (PE), and poly(ether ether ketone) (PEEK) were exposed to cultures of Staphylococcus epidermidis, Pseudomonas aeruginosa, or Escherichia coli. Bacteria washed and resuspended in phosphate buffered saline (PBS) adhered to polymers in amounts nearly twice those of bacteria that were left in their growth medium, tryptic soy broth (TSB). In TSB, there was variation in adhesion from species to species, but no significant variation from polymer to polymer within one species. In PBS there were significant differences in the amounts of bacteria adhering to the various polymers with the exception, of S. epidermidis, which had similar adhesion to all polymers. As a whole, P. aeruginosa was the most adherent while S. epidermidis was the least adherent. The estimated values of the free energy of adhesion (delta Fadh) correlated with the amount of adherent P. aeruginosa. When POE, PLA, and PSF were exposed to hyaluronic acid (HA) before exposure to the bacteria, there was 50% more adhesion of E. coli and P. aeruginosa on POE and PLA. With respect to bacterial adhesion, the biodegradable polymers (POE and PLA) in general were not significantly different from the nondegradable polymers.
将可降解聚合物聚原酸酯（POE）、聚-L-乳酸（PLA）以及不可降解聚合物聚砜（PSF）、聚乙烯（PE）和聚醚醚酮（PEEK）暴露于表皮葡萄球菌、铜绿假单胞菌或大肠杆菌的培养物中。用磷酸盐缓冲盐水（PBS）洗涤并重悬的细菌附着在聚合物上的数量几乎是留在其生长培养基胰蛋白胨大豆肉汤（TSB）中的细菌数量的两倍。在TSB中，不同菌种的粘附情况存在差异，但同一菌种内不同聚合物之间没有显著差异。在PBS中，除表皮葡萄球菌对所有聚合物的粘附情况相似外，不同聚合物上附着的细菌数量存在显著差异。总体而言，铜绿假单胞菌的粘附性最强，而表皮葡萄球菌的粘附性最弱。粘附自由能（ΔFadh）的估计值与附着的铜绿假单胞菌数量相关。当POE、PLA和PSF在暴露于细菌之前先暴露于透明质酸（HA）时，大肠杆菌和铜绿假单胞菌在POE和PLA上的粘附增加了50%。就细菌粘附而言，可降解聚合物（POE和PLA）总体上与不可降解聚合物没有显著差异。
