Corbel M J, Eades S M
J Hyg (Lond). 1976 Oct;77(2):221-33. doi: 10.1017/s0022172400024657.
Attempts were made to stimulate acquired immunity to experimental Absidia ramosa infection in mice. Unprotected animals inoculated with large doses of A. ramosa spores frequently developed acute phycomycosis of the central nervous system. Mice previously exposed to sub-lethal doses of spores showed a high resistance to subsequent challenge with A. ramosa. No consistent increase in resistance was observed in mice vaccinated with killed A. ramosa spores, hyphal walls, intracellular mycelial antigens or various combinations of these, with Freund's incomplete adjuvant. Antibodies to soluble mycelial antigens were inconsistently present in the sera of mice vaccinated with sub-lethal doses of viable spores. They were generally present in the sera of animals vaccinated with mycelial extracts of hyphal walls but not killed spores. Delayed hypersensitivity reactions to A. ramosa mycelial antigens could usually be elicited by intradermal tests in mice exposed to viable spores but irregularly in those vaccinated with non-viable preparations. Positive reactions were also frequently given by older mice not deliberately exposed to A. ramosa. Although mice previously exposed to viable A. ramosa spores were highly resistant to intravenous or intracerebral challenge with this fungus, they were more likely to develop persistent local granulomata on subcutaneous injection of spores than were unvaccinated animals.
人们尝试在小鼠中激发对实验性被孢霉感染的获得性免疫。接种大剂量被孢霉孢子的未受保护动物经常发生中枢神经系统急性藻菌病。先前暴露于亚致死剂量孢子的小鼠对随后的被孢霉攻击表现出高度抗性。在用灭活的被孢霉孢子、菌丝壁、细胞内菌丝抗原或这些物质与弗氏不完全佐剂的各种组合进行疫苗接种的小鼠中，未观察到抗性的持续增加。接种亚致死剂量活孢子的小鼠血清中，针对可溶性菌丝抗原的抗体存在情况不一致。它们通常存在于接种菌丝壁菌丝提取物而非灭活孢子的动物血清中。对被孢霉菌丝抗原的迟发型超敏反应通常可通过对暴露于活孢子的小鼠进行皮内试验引发，但在用无活力制剂接种的小鼠中则不规律。未特意暴露于被孢霉的老年小鼠也经常出现阳性反应。尽管先前暴露于活被孢霉孢子的小鼠对该真菌的静脉内或脑内攻击具有高度抗性，但与未接种疫苗的动物相比，它们在皮下注射孢子时更有可能形成持续性局部肉芽肿。
