COHN Z A
J Exp Med. 1963 Jan 1;117(1):43-53. doi: 10.1084/jem.117.1.43.
The influence of immune serum, PMN leucocytes, and macrophages from immunized animals and metabolic inhibitors on the intraphagocytic degradation of isotopically labeled bacteria has been evaluated. Immune serum specifically delayed the degradation of a variety of P(32)- and C(14)-labeled organisms within both types of phagocytic cells. The active principle in immune serum was found to be a globulin which could be removed by adsorption with the homologous organism. The inhibiting action of immune serum was thought to be related to its combination with the bacterial surface and the subsequent temporary protection of the bacteria from leucocyte enzymes. PMN leucocytes and macrophages obtained from immune hosts did not differ from normal cells in their ability to degrade homologous, labeled bacteria. Immune serum had the same inhibiting influence in the presence of "immune" cells as with cells from non-immunized hosts. Iodoacetate, arsenite, and cyanide at concentrations which inhibited the glycolysis and respiration of both PMN leucocytes and macrophages had no influence on the rate of degradation of isotopically labeled bacteria engulfed by these cells. This implied that following the initial phagocytic events, the degradation of bacteria within leucocytes is not dependent upon the major pathways of energy metabolism.
已评估免疫血清、免疫动物的中性粒细胞和巨噬细胞以及代谢抑制剂对同位素标记细菌吞噬内降解的影响。免疫血清特异性延迟了两种吞噬细胞内多种P(32)和C(14)标记生物体的降解。发现免疫血清中的活性成分是一种球蛋白，可通过与同源生物体吸附而去除。免疫血清的抑制作用被认为与其与细菌表面的结合以及随后对细菌的临时保护免受白细胞酶的作用有关。从免疫宿主获得的中性粒细胞和巨噬细胞在降解同源标记细菌的能力方面与正常细胞没有差异。在存在“免疫”细胞的情况下，免疫血清与来自未免疫宿主的细胞具有相同的抑制作用。碘乙酸盐、亚砷酸盐和氰化物在抑制中性粒细胞和巨噬细胞糖酵解和呼吸作用的浓度下，对这些细胞吞噬的同位素标记细菌的降解速率没有影响。这意味着在最初的吞噬事件之后，白细胞内细菌的降解不依赖于能量代谢的主要途径。
