Brett M, Findlay J B
Biochem J. 1979 Jan 1;177(1):215-23. doi: 10.1042/bj1770215.
The organization of rhodopsin in the photoreceptor membrane of sheep rod outer segments was investigated by using a variety of bifunctional reagents. Of the nine reagents used, seven gave oligomeric opsin species, whereas two, copper phenanthroline and dithiobisphenyl azide, failed to cross-link the protein. In general, the cross-linked species obtained showed diminishing yields from dimer to tetramer, together with some higher-molecular-weight aggregates. It is proposed that the patterns of cross-linking arise as a result of collision complexes and best describe a monomeric organization for native rhodopsin. No significant differences between the patterns obtained with dark-adapted bleached or regenerated protein states were observed. This interpretation is discussed in relation to the postulated mechanism of action of rhodopsin.
通过使用多种双功能试剂，对绵羊视杆细胞外段光感受器膜中视紫红质的组织进行了研究。在所使用的九种试剂中，七种产生了寡聚视蛋白物种，而另外两种，即邻菲罗啉铜和二硫代双苯叠氮化物，未能使蛋白质交联。一般来说，得到的交联物种从二聚体到四聚体的产率逐渐降低，同时还有一些更高分子量的聚集体。有人提出，交联模式是碰撞复合物的结果，最能描述天然视紫红质的单体组织。在暗适应、漂白或再生蛋白状态下获得的模式之间未观察到显著差异。结合视紫红质假定的作用机制对这一解释进行了讨论。
