正常细胞与转化细胞中的二胺氧化酶和多胺氧化酶活性

Diamine oxidase and polyamine oxidase activities in normal and transformed cells.

作者信息

Quash G, Keolouangkhot T, Gazzolo L, Ripoll H, Saez S

出版信息

Biochem J. 1979 Jan 1;177(1):275-82. doi: 10.1042/bj1770275.

DOI:10.1042/bj1770275

PMID:106846

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186366/

Abstract

The activity of diamine oxidase (EC 1.4.3.6) in normal rat kidney cells and in normal rat kidney cells transformed by avian sarcoma virus (B77 strain) growing in tissue culture varies with the stage of growth. There is an initial stimulation of activity by 24h after seeding, followed by a steep decline during exponential growth (48-72h). Enzyme activity decreases even further as the cells reach saturation density (confluence) after 4 days in culture when the activity in normal rat kidney cells is twice as high as that in transformed cells. 2. Differences of about the same order of magnitude are observed between transformed human cells HeLa, HEp2 (a human epithelioid carcinoma) and normal human fibroblasts, in chicken cells between normal myeloblasts and leukaemic myeloblasts, and in rats between biopsy material from normal mammary tissue and 9,10-dimethylbenz[a]anthracene-induced mammary tumours. 3. Polyamine oxidase activity also varies with the growth of transformed rat kidney cells, but shows no significant variation with the growth of normal rat kidney cells between 24 and 96h after seeding. The activity in cells at confluence is from 3- to 5-fold lower in the transformed than in the normal rat kidney cells. 4. A similar 5-10-fold decrease in activity has been found in 9,10-dimethylbenz[a]anthracene-induced mammary tumours in rats and in human oesophageal tumours. 5. Possible reasons for these observations and the contribution of these two enzymes to cellular putrescine concentrations are discussed.

摘要

在组织培养中生长的正常大鼠肾细胞以及被禽肉瘤病毒（B77株）转化的正常大鼠肾细胞中，二胺氧化酶（EC 1.4.3.6）的活性随生长阶段而变化。接种后24小时活性最初会受到刺激，随后在指数生长期（48 - 72小时）急剧下降。当培养4天后细胞达到饱和密度（汇合）时，酶活性进一步降低，此时正常大鼠肾细胞中的活性是转化细胞中的两倍。2. 在转化的人类细胞HeLa、HEp2（一种人类上皮样癌）与正常人类成纤维细胞之间，在鸡细胞的正常成髓细胞与白血病成髓细胞之间，以及在大鼠的正常乳腺组织活检材料与9,10 - 二甲基苯并[a]蒽诱导的乳腺肿瘤之间，观察到了大约相同数量级的差异。3. 多胺氧化酶活性也随转化的大鼠肾细胞的生长而变化，但在接种后24至96小时之间，正常大鼠肾细胞的生长过程中未显示出显著变化。汇合时转化细胞中的活性比正常大鼠肾细胞低3至5倍。4. 在大鼠的9,10 - 二甲基苯并[a]蒽诱导的乳腺肿瘤和人类食管肿瘤中，也发现了类似的活性降低5至10倍的情况。5. 讨论了这些观察结果的可能原因以及这两种酶对细胞腐胺浓度的贡献。