Bonnal C, Ravery V, Toublanc M, Bertrand G, Boccon-Gibod L, Hénin D, Grandchamp B
Department of Biochemistry B, Bichat Hospital, Paris, France.
Urology. 2000 Feb;55(2):287-91. doi: 10.1016/s0090-4295(99)00399-4.
To investigate the prevalence of the microsatellite instability related to mismatch repair (MMR) gene defects using a panel of six microsatellite markers, as recommended by a recent workshop on microsatellite instability in colon cancer, because it is still unclear whether abnormalities in DNA MMR genes are involved in transitional cell carcinoma (TCC) of the bladder.
Three mononucleotide repeats (BAT26, TGFbetaRII, and BAX) were studied in 33 TCC samples and in four bladder cancer cell lines. Three dinucleotide repeats (D2S123, D5S346, and D17S250) were studied in 21 of these 33 TCC samples.
No alteration was detected either in the 33 TCC samples analyzed or in the four bladder cancer cell lines (T24, J82, 647V, and 1207) studied. A difference between normal and tumor DNA was observed in only 1 of 21 tumor samples for D17S250.
These data indicate that microsatellite instability is very uncommon in TCC of the bladder.
按照近期一场关于结肠癌微卫星不稳定性研讨会的建议，使用一组六个微卫星标记物来研究与错配修复（MMR）基因缺陷相关的微卫星不稳定性的患病率，因为DNA错配修复基因异常是否参与膀胱移行细胞癌（TCC）仍不清楚。
在33例TCC样本和四种膀胱癌细胞系中研究了三个单核苷酸重复序列（BAT26、TGFbetaRII和BAX）。在这33例TCC样本中的21例中研究了三个二核苷酸重复序列（D2S123、D5S346和D17S250）。
在所分析的33例TCC样本以及所研究的四种膀胱癌细胞系（T24、J82、647V和1207）中均未检测到改变。在21例肿瘤样本中，仅1例样本的D17S250在正常DNA和肿瘤DNA之间观察到差异。
这些数据表明微卫星不稳定性在膀胱TCC中非常罕见。
