Nyberg F, Fransson J, Stephansson E
Department of Dermatology, Karolinska Hospital and Institute, Stockholm, Sweden.
Exp Dermatol. 2000 Feb;9(1):53-7. doi: 10.1034/j.1600-0625.2000.009001053.x.
Cutaneous lupus erythematosus (LE) often presents clinically as chronic cutaneous lesions, healing with scar formation, and acute cutaneous lesions that are seen in systemic and subacute LE and heal without scarring. UV-light plays a role in the pathogenesis of the skin lesions, but the pathomechanism is still unclear. The aim of this study was to compare fibroblast proliferation and response to UV-light by cultured fibroblasts from scarring and non-scarring LE lesions. Fibroblasts were cultured from skin lesions from 5 patients with classic discoid LE, 5 patients with subacute cutaneous LE and healthy, age-matched donors. Proliferation rate was assessed by cell counts at days 3, 6 and 9. The fibroblast cultures were irradiated with UVA and the supernatants were analysed for IL-6, TGF-beta, IL-4, soluble ICAM-1 and soluble VCAM-1. Fibroblast cultures from scarring lesions showed significantly lower cell-counts at days 3 (P = 0.01) and 9 (P = 0.009), than cultures from nonscarring lesions or controls. There were no significant differences in levels of IL-6 or TGF-beta in supernatants of irradiated fibroblasts from patients compared to controls and IL-4 and the soluble forms of ICAM-1 and VCAM-1 were below detection level. The response to UV-irradiation was similar to that of normal cells in the parameters studied. In summary, cultured fibroblasts from scarring LE lesions displayed significantly decreased proliferation rates compared to non-scarring LE lesions and controls. This may be secondary to inflammatory factors, or due to a functional defect in LE fibroblasts.
皮肤红斑狼疮（LE）临床上常表现为慢性皮肤损害，愈合后形成瘢痕，以及见于系统性和亚急性LE的急性皮肤损害，愈合后无瘢痕形成。紫外线在皮肤损害的发病机制中起作用，但其发病机制仍不清楚。本研究的目的是比较瘢痕性和非瘢痕性LE损害培养的成纤维细胞的增殖情况及对紫外线的反应。从5例经典盘状LE患者、5例亚急性皮肤LE患者和年龄匹配的健康供体的皮肤损害中培养成纤维细胞。在第3、6和9天通过细胞计数评估增殖率。用UVA照射成纤维细胞培养物，并分析上清液中的IL-6、TGF-β、IL-4、可溶性ICAM-1和可溶性VCAM-1。瘢痕性损害的成纤维细胞培养物在第3天（P = 0.01）和第9天（P = 0.009）的细胞计数显著低于非瘢痕性损害或对照的培养物。与对照相比，患者照射后的成纤维细胞上清液中IL-6或TGF-β水平无显著差异，且IL-4以及ICAM-1和VCAM-1的可溶性形式低于检测水平。在所研究的参数中，对紫外线照射的反应与正常细胞相似。总之，与非瘢痕性LE损害和对照相比，瘢痕性LE损害培养的成纤维细胞增殖率显著降低。这可能继发于炎症因子，或由于LE成纤维细胞的功能缺陷。
