Xu J, Ali R Y, Gregory D A, Amick D, Lambert S E, Yoell H J, Vilgalys R J, Mitchell T G
Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA.
Curr Microbiol. 2000 Apr;40(4):269-73. doi: 10.1007/s002849910053.
Restriction fragment length polymorphism (RFLP) in the large ribosomal RNA region of the mitochondrial DNA (mtDNA) was developed as a genetic marker for investigating mitochondrial transmission in sexual crosses of the human pathogenic basidiomycetous yeast Cryptococcus neoformans. Strain JEC20 of C. neoformans var. neoformans (mat a) was mated with six strains of C. neoformans var. grubii (mat alpha). Successful mating was indicated by the formation of hyphae and basidiospores. These basidiospores were examined for mtDNA RFLP genotypes. All 570 basidiospores examined from the six crosses showed the mtDNA genotype of strain JEC20. The failure to recover the C. neoformans var. grubii mtDNA in any cross indicates that the C. neoformans var. grubii mtDNA is either selectively eliminated in the newly formed dikaryon or selectively excluded in the immediate dikaryotic hyphae of the newly formed dikaryon.
线粒体DNA（mtDNA）大核糖体RNA区域的限制性片段长度多态性（RFLP）被开发为一种遗传标记，用于研究人类致病性担子菌酵母新型隐球菌有性杂交中的线粒体传递。新型隐球菌变种新型隐球菌（交配型a）的JEC20菌株与六种新型隐球菌变种格鲁比酵母（交配型α）菌株进行交配。菌丝和担孢子的形成表明交配成功。对这些担孢子进行了mtDNA RFLP基因型检测。从六个杂交组合中检测的所有570个担孢子均显示出JEC20菌株的mtDNA基因型。在任何杂交组合中均未回收新型隐球菌变种格鲁比酵母的mtDNA，这表明新型隐球菌变种格鲁比酵母的mtDNA要么在新形成的双核体中被选择性消除，要么在新形成的双核体的直接双核菌丝中被选择性排除。
