Han F, Ishiguro N, Ito T, Sakai T, Iwata H
Department of Orthopedic Surgery, Nagoya University School of Medicine, Japan.
Nagoya J Med Sci. 1999 Nov;62(3-4):115-26.
The aim of this study was to examine the effects of intraarticular administration of hyaluronan (HA) on cartilage degradation. Using a partial menisectomy model of osteoarthritis (OA) in the rabbit knee, the authors investigated the catabolic and anabolic changes induced by intraarticular injection of HA. To analyze anabolic changes, the authors assessed cell proliferation by measuring [3H] thymidine uptake, and proteoglycan biosynthesis by noting [35S] sulfate incorporation. For catabolic changes, messenger ribonucleic acid (mRNA) expression of interstitial collagenase (MMP-1), stromelysin-1 (MMP-3), and tissue inhibitor of metalloproteinase-1 (TIMP-1) in cartilage and synovium were detected with reverse transcriptase polymerase chain reaction (RT-PCR). Of significance for blocking the development of early OA in chondrocytes was the finding that total proteoglycan synthesis in the HA treatment group was significantly higher than in the controls. At the mRNA level in cartilage and synovium, HA inhibited MMP-3 and TIMP-1 production in the same way in the HA treatment group, while not affecting MMP-1 production. Thus it can be concluded that HA affects cartilage catabolism and anabolism to prevent the progress of OA.
本研究的目的是检测关节内注射透明质酸（HA）对软骨降解的影响。作者采用兔膝关节骨关节炎（OA）部分半月板切除术模型，研究关节内注射HA引起的分解代谢和合成代谢变化。为分析合成代谢变化，作者通过测量[3H]胸腺嘧啶核苷摄取评估细胞增殖，并通过记录[35S]硫酸盐掺入评估蛋白聚糖生物合成。对于分解代谢变化，采用逆转录聚合酶链反应（RT-PCR）检测软骨和滑膜中间质胶原酶（MMP-1）、基质溶解素-1（MMP-3）和金属蛋白酶组织抑制剂-1（TIMP-1）的信使核糖核酸（mRNA）表达。HA治疗组总蛋白聚糖合成显著高于对照组，这一发现对于阻止软骨细胞早期OA发展具有重要意义。在软骨和滑膜的mRNA水平上，HA治疗组中HA以相同方式抑制MMP-3和TIMP-1生成，而不影响MMP-1生成。因此可以得出结论，HA影响软骨的分解代谢和合成代谢，以防止OA进展。
