Saddik M, Lai R
Department of Pathology, College of Medicine, Royal University Hospital, Saskatoon, Canada.
J Clin Pathol. 1999 Nov;52(11):862-4. doi: 10.1136/jcp.52.11.862.
It has been shown that CD44 variants are differentially expressed in normal and neoplastic breast tissues. The diagnostic value of these markers in distinguishing benign from malignant breast lesions has not been well examined.
To evaluate the diagnostic value of CD44s in distinguishing between intraductal papillomas and papillary carcinomas of the breast, which may be difficult morphologically.
Expression of CD44s detected by immunohistochemistry was studied in a series of intraductal papillomas (11) and papillary carcinomas (10). The normal breast tissues surrounding the lesions of these cases served as a control. The number of CD44s positive epithelial cells was scored and categorised as < 10%, 10-70%, or > 70%.
Normal breast epithelial cells and all intraductal papillomas (11 of 11) expressed CD44s in a high proportion of cells (> 70%). In contrast, the majority of papillary carcinoma cases (eight of 10) expressed this marker in < 10% of the cells. In the remaining two papillary carcinoma cases, positivity was seen in more than 10% but still less than 70% of the cells.
CD44s detection by immunohistochemistry is useful in distinguishing intraductal papillomas from papillary carcinomas of the breast.
已有研究表明，CD44变体在正常乳腺组织和肿瘤性乳腺组织中存在差异表达。这些标志物在鉴别乳腺良性病变与恶性病变中的诊断价值尚未得到充分研究。
评估CD44s在鉴别乳腺导管内乳头状瘤和乳头状癌中的诊断价值，这两种病变在形态学上可能难以区分。
采用免疫组织化学方法检测了一系列乳腺导管内乳头状瘤（11例）和乳头状癌（10例）中CD44s的表达情况。将这些病例病变周围的正常乳腺组织作为对照。对CD44s阳性上皮细胞的数量进行评分，并分为<10%、10%-70%或>70%三类。
正常乳腺上皮细胞和所有导管内乳头状瘤（11/11）中，高比例细胞（>70%）表达CD44s。相比之下，大多数乳头状癌病例（10/8）中，该标志物在<10%的细胞中表达。其余2例乳头状癌病例中，阳性细胞比例超过10%但仍低于70%。
通过免疫组织化学检测CD44s有助于鉴别乳腺导管内乳头状瘤和乳头状癌。
