Lisovskaya I L, Shurkhina E S, Yakovenko E E, Tsvetaeva N V, Kolodei S V, Shcherbinina S P, Ataullakhanov F I
National Research Center for Hematology, Russian Academy of Medical Sciences, Moscow, Russia.
Biorheology. 1999;36(4):299-309.
We have previously proposed the osmofiltration method based on a modified Hanss hemorheometer to analyze distributions of erythrocytes in their ability to pass through membrane filters with 3 microns pores. Upon decrease in medium osmolality (u) the erythrocyte volume increases. When cell volume becomes V = Vcr at u = ucr, such cell loses its ability to pass through a 3 microns pore. The flow rate of erythrocyte suspension containing cells with different ucr through a filter gradually decreases with decreasing medium osmolality. This rate becomes zero at some u = omega, when the number of non-filterable cells in the applied sample approaches the number of pores in filter. Experimental determination of the dependencies of the filtration rate on medium osmolality for various hematocrit values allows to obtain omega for each hematocrit and, thereby, to assess the distribution of erythrocytes in ucr. Here, we propose a simplified version of this method, which allows screening of the erythrocytes in heterogeneous suspensions for the distribution in ucr by measuring omega for only two hematocrit values, 0.1% and 1%. Applications of the proposed method are exemplified by analysing the erythrocyte populations of healthy donors, of patients with microspherocytosis, hemochromatosis and normal erythrocyte populations in an acidic environment.
我们之前提出了一种基于改良汉斯血液流变仪的渗透过滤方法，用于分析红细胞通过3微米孔径膜过滤器的能力分布。随着介质渗透压（u）降低，红细胞体积增大。当细胞体积在u = ucr时变为V = Vcr，这样的细胞就失去了通过3微米孔径的能力。含有不同ucr的细胞的红细胞悬液通过过滤器的流速会随着介质渗透压降低而逐渐减小。当所施加样品中不可过滤细胞的数量接近过滤器中的孔数时，在某个u = ω时该流速变为零。通过实验确定不同血细胞比容值下过滤速率对介质渗透压的依赖性，能够为每个血细胞比容获得ω，从而评估红细胞在ucr中的分布。在此，我们提出了该方法的一个简化版本，通过仅测量0.1%和1%这两个血细胞比容值的ω，就能筛选异质悬浮液中的红细胞在ucr中的分布。通过分析健康供体、球形红细胞增多症患者、血色素沉着症患者以及酸性环境中正常红细胞群体的红细胞群体，举例说明了所提出方法的应用。
