Liuĭ Kh, Nefelova M V, Zakharchuk L M
M. V. Lomonosov Moscow State University.
Antibiot Khimioter. 2000;45(1):10-5.
The effect of various conditions of heat shock (1 hour at 35, 38, 40, 42, 45 and 50 degrees C) on the growth and morphological features of Streptomyces chrysomallus, an organism producing actinomycin, was studied. A definite regularity in the mycelium morphological changes at high temperatures was observed. After the shock at 35 and 38 degrees C the biomass volume and morphological features of the streptomycete did not markedly differ from those in the control. The shock at 40 degrees C induced the growth inhibition with decreasing the biomass volume by 50 per cent and appearance of submerged spores. When the shock conditions were more rigid (42, 45 and 50 degrees C) the mycelium growth lacked. It is of interest that the temperature of 42 degrees C induced abundant formation of the spores. With further increasing of the temperature to 45 and 50 degrees C the spore formation was not so abundant. The changes in the growth and development of the streptomycete are discussed in relation to the molecular mechanism of the cell protection from temperature shock.
研究了不同热休克条件（在35、38、40、42、45和50摄氏度下处理1小时）对产放线菌素的金色链霉菌生长和形态特征的影响。观察到高温下菌丝体形态变化具有一定规律性。在35和38摄氏度下热休克后，链霉菌的生物量体积和形态特征与对照相比无明显差异。40摄氏度的热休克导致生长抑制，生物量体积减少50%，并出现浸没孢子。当热休克条件更严格（42、45和50摄氏度）时，菌丝体生长停止。有趣的是，42摄氏度诱导了大量孢子形成。随着温度进一步升高至45和50摄氏度，孢子形成不那么丰富。结合细胞对温度休克的保护分子机制，讨论了链霉菌生长和发育的变化。
