Rho Y T, Lee K J
Department of Microbiology, Seoul National University, Korea.
Microbiology (Reading). 1994 Aug;140 ( Pt 8):2061-5. doi: 10.1099/13500872-140-8-2061.
We report the first quantitative analysis of the relationship between environmental changes and sporulation of a streptomycete, Streptomyces albidoflavus SMF301, in submerged culture. A chemically defined medium was constructed for sporulation, over 10(9) spores ml-1 being formed in the submerged batch culture. Kinetic parameters calculated from batch and chemostat cultures showed that specific submerged spore formation rate (qspo) was inversely related to the specific mycelial growth rate (mu). The optimum growth rate for submerged spore formation was 0.05 h-1, when the maximum value of qspo was 1.0 x 10(6) spores g-1 h-1. The turnover rate of biomass at maximum growth yield was 0.029 h-1, when 5.6 x 10(6) spores were formed from 1 g mycelium. The present quantitative analysis of submerged spore formation using a controlled system opens the way for biochemical and molecular biological studies related to the morphological differentiation of Streptomyces spp.
我们报道了在深层培养中，对环境变化与链霉菌（白色黄链霉菌SMF301）孢子形成之间关系的首次定量分析。构建了一种用于孢子形成的化学成分明确的培养基，在深层分批培养中形成了超过10⁹个孢子/毫升。从分批培养和恒化器培养计算得到的动力学参数表明，特定深层孢子形成速率（qspo）与特定菌丝体生长速率（μ）呈负相关。深层孢子形成的最佳生长速率为0.05 h⁻¹，此时qspo的最大值为1.0×10⁶个孢子/克·小时⁻¹。当从1克菌丝体形成5.6×10⁶个孢子时，最大生长产量下生物量的周转率为0.029 h⁻¹。目前使用控制系统对深层孢子形成进行的定量分析，为与链霉菌属形态分化相关的生化和分子生物学研究开辟了道路。
