Banerjee H N, Verma M, Hou L H, Ashraf M, Dutta S K
Department of Biology, and Cancer Research Center, Howard University, Washington, DC 20059, USA.
Yale J Biol Med. 1999 Jan-Feb;72(1):1-4.
The production and storage of explosives has resulted in the environmental accumulation of 2,4,6-trinitrotoluene (TNT). The biotransformation products of the nitroaromatic compound TNT and metabolites in mammalian cells in culture and their cytotoxicity are studied. We report after our analysis by reverse phase high performance liquid chromatography (HPLC) that the most prevalent biotransformation product of TNT in the NG108 neuroblastoma cells is primarily monoamino-dinitrotoluene (2Am-DNT). It causes toxic effects based on trypan blue exclusion and LDH-release colorimetric assays.
炸药的生产和储存导致了2,4,6-三硝基甲苯（TNT）在环境中的积累。对硝基芳香化合物TNT在培养的哺乳动物细胞中的生物转化产物、代谢物及其细胞毒性进行了研究。我们通过反相高效液相色谱（HPLC）分析后报告，在NG108神经母细胞瘤细胞中，TNT最普遍的生物转化产物主要是单氨基二硝基甲苯（2Am-DNT）。基于台盼蓝排斥法和乳酸脱氢酶（LDH）释放比色法，它会产生毒性作用。
