Dons L, Weclewicz K, Jin Y, Bindseil E, Olsen J E, Kristensson K
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
Med Microbiol Immunol. 1999 Aug;188(1):15-21. doi: 10.1007/s004300050100.
Neurotropism of Listeria monocytogenes was studied in rat dorsal root ganglia (DRG) and hippocampal neurons in culture. Using a system in which the DRG neurons can grow relatively free from other cells, it was observed that such DRG neurons, in contrast to hippocampal neurons, can be effectively infected by L. monocytogenes. The bacteria aligned along DRG axons, but not along hippocampal neurites. A mutant deficient in internalin, a protein required for entry into E-cadherin-expressing cells, did not interact with DRG neurons. Axonal migration of bacteria was studied in the DRG neurons grown in a double-chamber system, where either the neurites or the nerve cell bodies were exposed to the bacteria. The data suggest that L. monocytogenes can infect both axons and DRG nerve cell bodies, and that the bacteria can migrate in a retrograde as well as anterograde direction. These results support the notion that L. monocytogenes can spread via primary sensory neurons to the central nervous system. Infection of DRG primary sensory neurons, as employed in the present study, provides a model for analysis of bacterial and neuronal factors of importance for neurovirulence of L. monocytogenes.
在培养的大鼠背根神经节（DRG）和海马神经元中研究了单核细胞增生李斯特菌的嗜神经性。使用一种DRG神经元能够相对独立于其他细胞生长的系统，观察到与海马神经元不同，此类DRG神经元可被单核细胞增生李斯特菌有效感染。细菌沿DRG轴突排列，但不沿海马神经突排列。内化素是一种进入表达E-钙黏蛋白的细胞所需的蛋白质，缺乏内化素的突变体不与DRG神经元相互作用。在双室系统中生长的DRG神经元中研究了细菌的轴突迁移，在该系统中，神经突或神经细胞体暴露于细菌中。数据表明，单核细胞增生李斯特菌可感染轴突和DRG神经细胞体，且细菌可逆行和顺行迁移。这些结果支持单核细胞增生李斯特菌可通过初级感觉神经元传播至中枢神经系统这一观点。本研究中采用的DRG初级感觉神经元感染为分析对单核细胞增生李斯特菌神经毒力重要的细菌和神经元因素提供了一个模型。
