Schaade L, Kleines M, Walter R, Thomssen R, Ritter K
Department of Medical Microbiology, University Hospital, RWTH Aachen, Germany.
Med Microbiol Immunol. 1999 Aug;188(1):23-9. doi: 10.1007/s004300050101.
Gangliosides are known to influence cell growth and differentiation. The neolacto series ganglioside IV3NeuAc-nLc4 (2-->3-sialosylparagloboside) is present in members of the monocyte/granulocyte lineage, but is not found in cells that belong to the lymphocyte lineage. In this study we demonstrated that IV3NeuAc-nLc4 inhibits the proliferation of Epstein-Barr virus (EBV) genome-positive Burkitt lymphoma cells of the lines Raji and P3HR-1K. IV3NeuAc-nLc4-induced growth inhibition is associated with an increase in G0/G1 phase cells and a reduced expression of CD21 and HLA-DR antigens on Raji cells. These data suggest that IV3NeuAc-nLc4 may affect differentiation of lymphoma cells. Additionally, the increased expression of viral mRNA species which are characteristic for the lytic viral cycle in the non-producer line Raji and the enhanced release of virions from the producer line P3HR-1K demonstrate that IV3NeuAc-nLc4 activates the replication of EBV. Growth inhibition and termination of the viral latency suggest that IV3NeuAc-nLc4 present in monocyte/granulocyte lineage cells may be an effector of the natural defense against EBV persistency and transformation.
已知神经节苷脂会影响细胞生长和分化。新乳糖系列神经节苷脂IV3NeuAc-nLc4（2→3-唾液酸基副球蛋白）存在于单核细胞/粒细胞谱系的成员中，但在属于淋巴细胞谱系的细胞中未发现。在本研究中，我们证明IV3NeuAc-nLc4可抑制Raji和P3HR-1K系的爱泼斯坦-巴尔病毒（EBV）基因组阳性伯基特淋巴瘤细胞的增殖。IV3NeuAc-nLc4诱导的生长抑制与G0/G1期细胞增加以及Raji细胞上CD21和HLA-DR抗原表达降低有关。这些数据表明IV3NeuAc-nLc4可能影响淋巴瘤细胞的分化。此外，非生产性系Raji中裂解病毒周期特有的病毒mRNA种类表达增加以及生产性系P3HR-1K中病毒粒子释放增强，表明IV3NeuAc-nLc4可激活EBV的复制。生长抑制和病毒潜伏期的终止表明，单核细胞/粒细胞谱系细胞中存在的IV3NeuAc-nLc4可能是针对EBV持续性和转化的天然防御的效应物。
