Martínez M A, Ovalle A, Ulloa M T, Vidal R M
Universidad de Chile, Facultad de Medicina, Instituto de Ciencias Biomédicas, Programa de Microbiología, Santiago.
Eur J Clin Microbiol Infect Dis. 1999 Dec;18(12):890-2. doi: 10.1007/s100960050425.
Haemophilus spp. were isolated from the amniotic fluid of eight of 110 consecutive women with preterm premature rupture of membranes (PROM) between 1992 and 1998. Isolates were nontypeable and classified according to biochemical test results as Haemophilus influenzae biotype I (n = 1), biotype II (n = 4), biotype III (n = 1) or biotype IV (n = 2). Primers recognizing specific sequences in the 16S rRNA of the cryptic genospecies of Haemophilus were employed to amplify the DNA of the eight isolates. One isolate classified as Haemophilus influenzae biotype II was confirmed as belonging to the genital cryptic species. Infectious morbidity occurred in five women and two newborns and was associated in most cases with biotype II.
1992年至1998年间，在110例连续发生胎膜早破（PROM）的孕妇中，有8例孕妇的羊水分离出嗜血杆菌属。分离株不可分型，根据生化检测结果分类为流感嗜血杆菌生物I型（n = 1）、生物II型（n = 4）、生物III型（n = 1）或生物IV型（n = 2）。使用识别嗜血杆菌隐性基因种16S rRNA中特定序列的引物扩增8株分离株的DNA。1株分类为流感嗜血杆菌生物II型的分离株被确认为属于生殖道隐性种。5名女性和2名新生儿发生了感染性发病，大多数病例与生物II型有关。
Zotero 插件