Quentin R, Martin C, Musser J M, Pasquier-Picard N, Goudeau A
Département de Microbiologie Médicale et Moléculaire, Centre Hospitalier, Universitaire Bretonneau, Tours, France.
J Clin Microbiol. 1993 May;31(5):1111-6. doi: 10.1128/jcm.31.5.1111-1116.1993.
In recent years, reports originating from several areas of the world have identified biotype IV strains of Haemophilus influenzae as a cause of serious urogenital, neonatal, and mother-infant infections. Preliminary analysis of a sample of biotype IV isolates found evidence for a cryptic genospecies of Haemophilus (R. Quentin, A. Goudeau, R. J. Wallace, Jr., A. L. Smith, R. K. Selander, and J. M. Musser. J. Gen. Microbiol. 136:1203-1209, 1990). Eighteen biotype IV strains assigned to the cryptic genospecies were further characterized by their rDNA restriction fragment length polymorphism patterns and genomic DNA-DNA hybridization. Isolates of the cryptic genospecies have distinctive rDNA restriction fragment length polymorphisms that differ from those of Haemophilus haemolyticus and H. influenzae. Genomic hybridization studies show that these organisms are allied with H. influenzae and H. haemolyticus and suggest a distant trifurcation of H. influenzae, H. haemolyticus, and the cryptic genospecies.
近年来，来自世界多个地区的报告已确认，流感嗜血杆菌生物IV型菌株是严重泌尿生殖系统、新生儿及母婴感染的病因。对生物IV型分离株样本的初步分析发现了流感嗜血杆菌一个隐秘基因种存在的证据（R. 昆廷、A. 古多、小R. J. 华莱士、A. L. 史密斯、R. K. 塞兰德和J. M. 马瑟。《普通微生物学杂志》136:1203 - 1209, 1990年）。通过其核糖体DNA限制片段长度多态性图谱和基因组DNA - DNA杂交，对归入该隐秘基因种的18株生物IV型菌株进行了进一步特性分析。该隐秘基因种的分离株具有独特的核糖体DNA限制片段长度多态性，与溶血嗜血杆菌和流感嗜血杆菌的不同。基因组杂交研究表明，这些微生物与流感嗜血杆菌和溶血嗜血杆菌相关联，并提示流感嗜血杆菌、溶血嗜血杆菌和该隐秘基因种存在远源的三分歧。
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