两种用于从临床标本中分离分枝杆菌的自动化系统的比较。

Comparison of two automated systems for the isolation of mycobacteria from clinical specimens.

作者信息

Flanagan P G, Williams R, Paull A

机构信息

Regional Centre for Mycobacteriology, Cardiff Public Health Laboratory, University Hospital of Wales, Heath Park, UK.

出版信息

Eur J Clin Microbiol Infect Dis. 1999 Dec;18(12):912-4. doi: 10.1007/s100960050431.

DOI:10.1007/s100960050431

PMID:10691207

Abstract

The Bactec MGIT 960 (Becton-Dickinson, UK) automated mycobacterial liquid culture system was compared with the Bactec 9000 MB (Becton-Dickinson) in order to assess ease of use, diagnostic reliability and safety features. One thousand twenty-nine clinical specimens were cultured in parallel, yielding a total of 125 (12.1%) mycobacterial isolates, including 71 Mycobacterium tuberculosis and 18 Mycobacterium avium. The Bactec MGIT 960 demonstrated a mycobacterial recovery rate and speed of detection equivalent to that of the Bactec 9000 MB for clinically important isolates. The Bactec MGIT 960 integrates smoothly into laboratory workflow, does not require needle inoculation and has a much larger capacity than the Bactec 9000 MB.

摘要

为评估易用性、诊断可靠性和安全特性，将Bactec MGIT 960（英国BD公司）自动化分枝杆菌液体培养系统与Bactec 9000 MB（BD公司）进行了比较。对1029份临床标本进行平行培养，共获得125株（12.1%）分枝杆菌分离株，其中包括71株结核分枝杆菌和18株鸟分枝杆菌。对于具有临床重要性的分离株，Bactec MGIT 960的分枝杆菌回收率和检测速度与Bactec 9000 MB相当。Bactec MGIT 960能顺利融入实验室工作流程，无需针式接种，且容量比Bactec 9000 MB大得多。