通过免疫亲和柱色谱法从人参中分离出具有药理活性的皂苷人参皂苷Rb1。
Isolation of the pharmacologically active saponin ginsenoside Rb1 from ginseng by immunoaffinity column chromatography.
作者信息
Fukuda N, Tanaka H, Shoyama Y
机构信息
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
出版信息
J Nat Prod. 2000 Feb;63(2):283-5. doi: 10.1021/np990356s.
PMID:10691730
Abstract
Immunoaffinity column chromatography using an anti-ginsenoside Rb1 monoclonal antibody has made possible a single-step separation of ginsenoside Rb1 from a crude extract of ginseng roots (Panax ginseng). The combination of immunoaffinity column chromatography and an enzyme-linked immunosorbent assay (ELISA) was also investigated.
摘要
使用抗人参皂苷Rb1单克隆抗体的免疫亲和柱色谱法已使从人参根（人参）粗提物中一步分离人参皂苷Rb1成为可能。还研究了免疫亲和柱色谱法与酶联免疫吸附测定法（ELISA）的结合。
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